TY  - JOUR
T1  - Ethanol Extract of Pinus nigra ssp. pallasiana var. şeneriana Inhibits Human Breast Cancer Cell Viability through Induction of Apoptosis
AU  - Atmaca, Harika
AU  - Çamlı, Çisil
AU  - Sert, Seda
PY  - 2018
DA  - March
DO  - 10.18466/cbayarfbe.334883
JF  - Celal Bayar University Journal of Science
JO  - CBUJOS
PB  - Manisa Celal Bayar Üniversitesi
WT  - DergiPark
SN  - 1305-130X
SP  - 35
EP  - 40
VL  - 14
IS  - 1
LA  - en
AB  - Novel natural compounds became the new promising source for alternativedrug production for cancer treatment. Several studies have reported thatdifferent species of pine genus show cytotoxic activities against variouscancer cell lines. In the present study, the possible cytotoxic and apoptoticeffects of the endemic Pinus nigra ssp. pallasianavar. şeneriana (PS) extract inMCF-7 breast cancer cells were evaluated. Cell viability was determined via XTT assay and verified by live/deadviability/cytotoxicity assay. Apoptotic effect was investigated bymitochondrial membrane potential (MMP) assay. Expression of apoptosis relatedproteins after treatment with PS extract was detected by apoptosis array. PSextract treatment reduced the viability of MCF-7 cells in a time andconcentration-dependent manner. Apoptosis was induced and MMP was decreased inMCF-7 cells by 48 h treatment. Bcl-2, cIAP-1,cIAP-2, Survivin and XIAP protein levelswere reduced by 2.8-, 3.2-, 2.8-, 2.5- and 2.2- fold, and Bad, Bax, Cytochromec and SMAC/Diablo protein levels were induced by 4.2-, 3.0-, 3.6-, and 4.0-fold in MCF-7 cells by PS extract treatment. This report describes thecytotoxic and apoptotic effects of the extract of endemic Pinus species onMCF-7 cells for the first time. Althoughthe isolation and structure elucidation of the active compounds is needed to beperformed in the further studies, it is likely that PS extract may be apotential candidate for the development of novel therapeutic anticancercompounds for breast cancer.
KW  - Apoptosis
KW  - breast cancer
KW  - cytotoxicity
KW  - endemic
KW  - MCF-7
KW  - Pinus nigra
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UR  - https://doi.org/10.18466/cbayarfbe.334883
L1  - https://dergipark.org.tr/tr/download/article-file/447385
ER  -