@article{article_336398, title={The Protective Effect of Iloprost and Low-Dose Methotrexate on Testicular Ischemia/Reperfusion Injury in an Experimental Model}, journal={The Journal of Kırıkkale University Faculty of Medicine}, volume={19}, pages={131–138}, year={2017}, DOI={10.24938/kutfd.336398}, author={Yuvanç, Ercan and Tuğlu, Devrim and Kısa, Üçler and Bozdoğan, Önder and Batislam, Ertan and Yılmaz, Erdal}, keywords={Testis torsiyonu,detorsiyon,antioksidan,iskemi/reperfüzyon,iloprost,düşük doz metotreksat}, abstract={<p class="MsoNoSpacing" style="margin-bottom:3pt;text-align:justify;line-height:115%;"> <b> <span style="font-size:9pt;line-height:115%;">Objective </span> </b> <span style="font-size:9pt;line-height:115%;">:  </span> <span style="font-size:9pt;line-height:115%;">To investigate the protective effects of iloprost and
low-dose methotrexate on ischemia/reperfusion (I/R) injury in surgically
detorsed testicular torsion. </span> <span style="font-size:9pt;line-height:115%;"> </span> </p> <p> </p> <p class="MsoNoSpacing" style="margin-bottom:3pt;text-align:justify;line-height:115%;"> <b> <span style="font-size:9pt;line-height:115%;">Material and Methods: </span> </b> <span style="font-size:9pt;line-height:115%;"> </span> <span style="font-size:9pt;line-height:115%;">Rats were randomly divided into 4 groups: Group 1 (sham),
Group 2: Only testicular I/R; Group 3: I/R+iloprost (10 μg/kg) and Group 4:
I/R+low dose metotrexate (6 mg/kg). Torsion / ischemia and detorsion /
reperfusion times were determined as 4 hours. Medications were given
intraperitoneally at 3 hours following onset of testicular torsion. Detorsioned
right testises were removed 8 hours after the begining of the procedure in all
4 groups. </span> <span style="font-size:9pt;line-height:115%;"> </span> </p> <p> </p> <p class="MsoNoSpacing" style="margin-bottom:3pt;text-align:justify;line-height:115%;"> <b> <span style="font-size:9pt;line-height:115%;">Results: </span> </b> <span style="font-size:9pt;line-height:115%;"> </span> <span style="font-size:9pt;line-height:115%;">In our study, we found that administration of iloprost and
metotrexate significantly increased total antioxidant capacity (TAC) while
reducing total oxidant statüs (TOS) and oxidative stress index (OSI) levels
significantly. No statistically significant difference was found for testicles
in I/R groups according to the Johnsen scoring system. </span> <span style="font-size:9pt;line-height:115%;"> </span> </p> <p> </p> <p> </p> <p class="MsoNoSpacing" style="margin-bottom:3pt;text-align:justify;line-height:115%;"> <b> <span style="font-size:9pt;line-height:115%;">Conclusion: </span> </b> <span style="font-size:9pt;line-height:115%;"> </span> <span style="font-size:9pt;line-height:115%;">Admnistration of iloprost and low dose methotrexate treatment
increased antioxidant activity in testicular samples while suppressing oxidant
activity. However, there was no change in histopathological examination and
spermatogenesis scores. </span> <span style="font-size:9pt;line-height:115%;"> </span> </p> <p> </p>}, number={3}, publisher={Kırıkkale Üniversitesi}