@article{article_344127, title={A five-year retrospective evaluation of t(4;11) MLL/AF4 translocation results with real time RT-PCR in acute lymphoblastic leukemia patients}, journal={Ege Journal of Medicine}, volume={54}, pages={107–110}, year={2015}, DOI={10.19161/etd.344127}, author={Süslüer, Sunde YILMAZ and Kaymaz, Burçin Tezcanli and Çetintaş, Vildan Bozok and Vardarlı, Aslı Tetik and Aygüneş, Duygu and Dalmızrak, Ayşegül and Aktan, Çağdaş and Küçükaslan, Ali Şahin and Balcı, Tuğçe and Kayabaşı, Çağla and et al.}, keywords={t(4;11) MLL-AF4 translokasyonu, gerçek zamanlı RT-PCR}, abstract={Aim: t(4,11) is a chromosomal abnormality formed by the translocation MLL-AF4, which is the result of the fusion of the AF4 gene, localized on 4q21 chromosomal band, to the MLL gene, localized on 11q23 chromosomal band. The aim of this study is to examine the results of the analysis of t (4;11) MLL-AF4 translocation in acute lymphoblastic leukemia (ALL) patients retrospectively. Materials and Methods: Peripheral blood or bone marrow samples of 176 children (70 girls, 106 boys) and 144 adults (60 women, 84 men) with a preliminary diagnosis of acute leukemia between 2009-2013 were analyzed in the Medical Biology Department of Ege University Faculty of Medicine. The translocation RNA results of 71 peripheral blood and 473 bone marrow samples of these patients were evaluated quantitatively for t(4;11) with real-time RT-PCR. t(4;11) quantitation was performed by real-time qRT-PCR instrument after the synthesis of complementary DNA with conventional PCR from total RNA or mRNA isolated from blood and bone marrow. Quantitative analysis of the patients was performed by comparing positive and negative controls and samples classified as positive or negative (the ratio of the number of positive copies to the number of reference copies). Results: A total of 320 patients, with 98 having also follow-ups, were evaluated for t(4;11) translocation. Totally 34 patients (24 children and 10 adults) were found positive and the other samples were negative. Conclusion: The assessment of these results supports that, quantitative determination of t(4;11) with RT-PCR method among newly diagnosed ALL patients and ALL patients undergoing treatment, is a valuable method for both confirming the diagnosis and guiding the treatment intended to achieve molecular remission.}, number={3}, publisher={Ege Üniversitesi}