TY - JOUR TT - Cell-type specific analysis: The use of fluorescence-activated cell sorting (FACS) in Arabidopsis thaliana leaves AU - Gören Sağlam, Nihal PY - 2017 DA - December Y2 - 2017 DO - 10.7240/marufbd.371519 JF - Marmara Fen Bilimleri Dergisi JO - MFBD PB - Marmara Üniversitesi WT - DergiPark SN - 2146-5150 SP - 131 EP - 133 VL - 29 IS - 4 KW - FACS KW - GFP KW - Arabidopsis thaliana KW - gen aktivitesi N2 - Cell-specific resolution of gene activity iscritical to understand specific developmental events. However, such highresolution data have been difficult to obtain at a genomic level becausespecific types of cell need to be isolated. One of the most efficient method toisolate specific cells from neighboring cells in the same organ or tissue isFACS. Theisolated cells can be used for genomic analysis. In this review, it will begiven information about FACS in transgenic Arabidopsisthaliana leaves which are labelled with GFP. CR - Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Galbraith D.W., Benfey P.N., 2003, A gene expression map of the Arabidopsis root. Science, 302:1956-1960 CR - Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Hirst, J.A., Galbraith D.W., Benfey P.N., 2005, Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent repeater lines. Nat. Methods., 2:615-619 CR - Carter A.D., Bonyadi R., Gifford M.L., 2013, The use of fluorescence-activated cell sorting in studying plant development and environmental responses. Int. J. Dev. Biol., 57:545-552 CR - Gronlund J.T., Eyres A., Kumar S., Buchanan-Wollaston V., Gifford M.L., 2012, Cell specific analysis of Arabidopsis leaves using flourescence activated cell sorting, J. Vis. Exp. (68), e4214 10.3791/4214 DOI: 10.37914214 (2012) CR - Hertberg M., Sievertzaon M., Aspeborg H., Nilsson P., Sandberg G., Lundeberg J., cDNA microarray analysis of small plant tissue samples using cDNA target amplification protocol. Plant J., 25:585-591 CR - Lee J.Y., Levesque M., Benfey P.N., 2005, High-throughput RNA isolation technologies. New tools for high-resolution gene expression profiling in plant systems. Plant Physiol., 138:585-590 CR - Wang Y. and Jiao Y., 2011, Advances in plant cell type-spesific genome-wide studies of gene expression. Front. Biol., 6:384-389 UR - https://doi.org/10.7240/marufbd.371519 L1 - https://dergipark.org.tr/tr/download/article-file/438126 ER -