TY  - JOUR
T1  - The Effects of Titanium Dioxide Nanoparticles on Vitality and Ultrastructure of Yac-1 Lymphoma Cell Line
TT  - Titanyum Dioksit Nanoparçacıklarının Yac-1 Lenfoma Hücre Soyu Canlılığı Ve İnce Yapısı Üzerine Etkileri
AU  - Kotil, Tuğba
AU  - Erdoğan, Aslı
AU  - Mutlu, Hasan Serdar
AU  - Doğan, Sibel
AU  - Solakoğlu, Seyhun
PY  - 2018
DA  - March
DO  - 10.18017/iuitfd.373670
JF  - Journal of Istanbul Faculty of Medicine
JO  - İst Tıp Fak Derg
PB  - İstanbul Üniversitesi
WT  - DergiPark
SN  - 1305-6441
SP  - 17
EP  - 24
VL  - 81
IS  - 1
LA  - en
AB  - Objective: Nanoparticles, which are widelyused, are known to be effective in cancer therapy. The aim of this study is toinvestigate the effects of titanium dioxide nanoparticles (TiO2-NP) on theYAC-1 lymphoma cell line.Materials and Methods: TiO2-NP were appliedat two different concentrations (0.1mg/mL, 0.5mg/ mL) on cultured YAC-1lymphoma cells in 24 well plates. After 24 and 48 hours of treatment the cellswere collected and counted for viability test and the quantitative differencewas determined by the Tukey-Kramer multiple comparison test. Cells wereembedded in Epon-812 after routine process for the electron microscopeexamination. Thin sections of the cells were examined with Jem Jeoltransmission electron microscope.Results: The proliferation index of YAC-1cells showed a statistically significant decrease with the dose and timedependent of TiO2-NP treatment. Mitochondrial degeneration, autophagic vacuoleaccumulation and both necrotic and apoptotic cell morphologies were observed inboth TiO2-NP treatment groups via electron microscopic evaluation. Conclusion: Several studies have shown thatTiO2-NPs trigger cell death on different cancer cell lines. In our study, weobserved autophagic vacuole filled with NPs, organelle degeneration, andmorphological changes showing apoptotic and necrotic cell death in groupsexposed to nanoparticles. In addition significant decrease of proliferationindex were detected with dose- and time-dependent. We are constantlyinteracting with TiO2-NP in our daily day life. The mechanisms of thesecytotoxic effects of TiO2-NP should be elucidated by further studies.
KW  - Nanoparticles
KW  - titanium dioxide
KW  - YAC-1
KW  - autophagy
KW  - apoptosis
N2  - Amaç: Yaygın kullanım alanı olan nanoparçacıklarınkanser terapisinde etkili oldukları bilinmektedir. Çalışmamızda geniş kullanımalanına sahip titanyum dioksit nanoparçacıklarının (TiO2-NP) YAC-1 lenfomahücre soyu üzerine etkilerini incelemeyi amaçladık.Gereç ve Yöntem: 24’lü kültür kuyucuklarınaekilen YAC-1 lenfoma hücreleri üzerine iki (0,1mg/mL, 0,5mg/mL) farklıkonsantrasyonda TiO2-NP’si uygulandı. 24 ve 48 saat sonra hücreler toplanarakcanlılık testi için sayma kamarasında sayım yapıldı ve nicel farklılıkTukey-Kramer çoklu karşılaştırma testi ile belirlendi. Elektron mikroskop incelemesiiçin rutin takip sonrası Epon-812 ortamına gömülen hücrelerden alınan incekesitler geçirimli elektron mikroskobu ile incelendi. Bulgular: YAC-1 hücrelerinin çoğalmaindekslerinde TiO2-NP’lerin dozuna ve uygulama süresine bağlı olarak istatistikselolarak anlamlı düzeyde azalma izlendi. Elektron mikroskobik incelemede, 24.saatte 0,5mg uygulanmış grupta ve 48. saatte 0,1 ve 0,5 mg uygulanmış gruptamitokondri bozulması, otofajik vakuol birikimi ve hem nekrotik hem de apoptotikhücre morfolojilerine rastlandı. Sonuç: TiO2-NP’lerinin farklı kanser hücresoyları üzerinde hücre ölümünü tetiklediği çeşitli çalışmalarla gösterilmiştir.Çalışmamızda hücre çoğalma indeksi açısından doza ve süreye bağlı azalmaya ekolarak elektron mikroskobik olarak nanoparçacığa maruz kalan hücrelerde TiO2-NPiçeren otofajik vakuol oluşumları, organellerin yapısında bozulmalar veapoptotik ve nekrotik hücre ölümünü gösteren morfolojik değişimleri izledik.Çevremizde sürekli etkileşim halinde olduğumuz bu TiO2-NP’sinin hücre üzerindegösterdiği bu sitotoksik etkinin mekanizmaları, kapsamlı araştırmaların konusuolarak değerlendirilmelidir.
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UR  - https://doi.org/10.18017/iuitfd.373670
L1  - https://dergipark.org.tr/tr/download/article-file/453623
ER  -