TY  - JOUR
TT  - Identification and Pathogenicitiy of Botryosphaeriaceae Species Causing Wood Canker on Grapevines
AU  - Akgül, D. Soner
AU  - Güngör Savaş, Nurdan
AU  - Teker, Turcan
PY  - 2014
DA  - May
JF  - The Journal of Turkish Phytopathology
PB  - Türkiye Fitopatoloji Derneği
WT  - DergiPark
SN  - 0378-8024
SP  - 35
EP  - 45
VL  - 42
IS  - 1-2-3
KW  - Botryosphaeriaceae
KW  - Grapevine
KW  - Trunk Diseases
N2  - Woodcanker and dieback diseases, caused by Botryosphaeriaceae fungi, are among themost important fungal trunk diseases of grapevines. In recent years, thesymptoms of local dead arm, shoot dieback and V-shaped discolorations in woodytissues have dramatically increased in vines of Turkey. During the 2012 growingseason, symptomatic wood samples (from Ankara, Çorum, Izmir, and Manisa cities)were taken and standard mycological isolations were done to determine thefungal agents of disease. A high proportion (69.4%) of Botryosphaeriaceae fungiwas isolated from these samples. In the first stage of the isolates’identification,fungal DNA was extracted and amplified in Real-Time thermocycler by using thegenus spesific primers (BOT100F-BOT472R). In this way, the members ofBotryosphaeriaceae species were distinguished from other similar species. Inmolecular identification of the isolates, ITS 1 and ITS 2 ribosomal DNAfragments were sequenced and the gene sequences were compared with thosedeposited in NCBI Gene Bank database.It has been determined that 4 differentspecies Botryosphaeria dothidea (Anamorph;Fusicoccum aesculi), Diplodia seriata(An; B. obtusa) Lasiodiplodia theobromae (An; B.rhodina) andNeofusicoccum parvum(An; B. parva) were found in thesequenced 15 isolates. Colony morphology on PDA media andpicniospore shapes onwoody tissues were examined in morphological / microscopic identification. Tofulfill Koch’s postulates, pathogenicity tests for all isolates were conductedon 1-year-old potted vines. Four months after inoculation, the lesion lengthson woody tissues were evaluated. Pathogenicity tests revealed that Neofusicoccum parvum species producedlarger lesions on woody tissues when compared to control and other species.
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UR  - https://dergipark.org.tr/tr/pub/fitopatoloji/article/382472
L1  - https://dergipark.org.tr/tr/download/article-file/409235
ER  -