TY  - JOUR
TT  - The identification of virus diseases in olive trees in Aydin, Balikesir and İzmir provinces and the determination of their present status
AU  - Erilmez, Serpil
AU  - Erkan, Semih
PY  - 2014
DA  - March
JF  - Plant Protection Bulletin
PB  - TARIM VE ORMAN BAKANLIĞI EĞİTİM VE YAYIN DAİRESİ BAŞKANLIĞI
WT  - DergiPark
SN  - 0406-3597
SP  - 45
EP  - 67
VL  - 54
IS  - 1
KW  - Zeytin
KW  - virüs
KW  - DAS-ELISA
KW  - RT-PCR
KW  - biyolojik indeksleme
N2  - The present study was carried out to determine the presence andprevalence of the viral agents in olive production areas of Aydın, Balıkesir andIzmir in 2009-2010. In 2009, totally 293 samples were collected from Aydın(157) and Izmir (136) provinces. In 2010, with collected 82 samples fromBalıkesir, the total sample number in the research has reached to 375. Toidentify viral agents, the biological, serological and molecular methods wereapplied to collected olive samples. DAS-ELISA method was used to detect Arabismosaic virus (ArMV), Cucumis mosaic virus (CMV), Cherry leafrollvirus (CLRV) and Strawberry latent ringspot virus (SLRSV). Also, theexistence of Olive latent-1 virus (OLV-1), Olive latent-2 virus(OLV-2), Olive latent-3 virus (OLV-3), Olive latent ringspot virus(OLRSV) and Olive leaf yellowing-associated virus (OLYaV) in the sampleswas analysed by RT-PCRmolecular method. The results of RT-PCR analyses showed that the incidence ofvirus infection was 55.73%in total 375 olive samples from Aydın, Balıkesir andIzmir in 2009 and 2010. According to individual provinces, it was 22.66% inAydın, 11.46 in Balıkesir and 21.60% in Izmir. DAS-ELISA results revealed that infection rate of ArMV was 12.8% intotal samples and only 1 sample was found to be infected with SLRSV while othertested viruses were not present. According to the data from RT-PCR tests,infection rates in all tested samples were 22.93%, 9.60%, 10.66% and 9.06% forArMV, CMV, CLRV and SLRSV, respectively. Moreover, it was ascertained that 9samples were infected by CMV + CLRV and 4 samples by ArMV + CLRV as mixedinfections. Other viruses including OLV-1, OLV-2, OLV-3, OLRSV and OLYaV werenot detected in all samples.  
CR  - Cardoso J.M.S., Felix M.R.,Clara M.I.E and Oliveira S. 2005. The complete genome sequence of a new necrovirus isolated from Olea europea L. Archives of Virology, 150: 815-823.
UR  - https://dergipark.org.tr/tr/pub/bitkorb/article/382987
L1  - https://dergipark.org.tr/tr/download/article-file/409945
ER  -