@article{article_386041, title={Mass Spectrometric Quantitation of Serum Cephazolin}, journal={The Turkish Journal Of Occupational / Environmental Medicine and Safety}, volume={2}, pages={61–61}, year={2017}, author={Unlu, Ali and Abusoglu, Sedat and Sivrikaya, Abdullah}, keywords={Mass Spectrometric Quantitation of Serum Cephazolin}, abstract={<p class="MsoNormal" style="text-align:justify"> <b> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Objectives </span> </b> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif""> <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Cefazolin, a first generation cephalosporin </span> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif"; mso-bidi-font-family:Arial">β </span> <span style="mso-bidi-font-size:9.0pt; font-family:"Calibri","sans-serif"">-lactam antibiotic, is commonly used for prophylaxis during cesarean delivery, and in treatment of pulmonary infections and many surgical procedures. The aim of this work was to develop a simple, fast and accurate liquid-chromatography-mass spectrometry method for determination of serum cefazolin. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif""> <o:p>  </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <b> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Methods </span> </b> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif""> <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Mass spectrometric analyses were performed using an Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API 3200 triple quadrupole mass spectrometer (USA) equipped with an electrospray ion source (ESI) operating in positive mode. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <b> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Results </span> </b> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif""> <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">The standard curves for cefazolin was linear within the range of 0.39-200 µg/ml. Total run time was 5 minutes. Chromatographic separation was performed on a C18 column <st1:citation w:st="on">(4.6×50 mm, 5 µm, Phenomenex Luna) </st1:citation> with a mobile phase consisting of 1% formic acid in water and MeOH <st1:citation w:st="on">(10:90, V/V) </st1:citation> at a flow rate of 0.8 ml/min. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif""> <o:p>  </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <b> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Discussion </span> </b> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif""> <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Serum cefazolin measurement can be easily performed by LC-MS/MS system to identify the risk of the patients with therapeutic drug monitoring. This method performs this drug analysis with high throughput. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif";mso-bidi-font-family:Arial"> <o:p>  </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <b> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif"">Conclusions <o:p> </o:p> </span> </b> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Exact determination of cefazolin provides reliable levels in clinical studies to avoid toxic effects. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif""> <o:p>  </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <b> <span style="mso-bidi-font-size:9.0pt;font-family:"Calibri","sans-serif"">Keywords <o:p> </o:p> </span> </b> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif"">Antibiotic levels, mass spectrometry, cefazolin, toxicity. <o:p> </o:p> </span> </p> <p class="MsoNormal" style="text-align:justify"> <span style="mso-bidi-font-size: 9.0pt;font-family:"Calibri","sans-serif""> <o:p>  </o:p> </span> </p> <p>}, number={1(4)}, publisher={Engin TUTKUN}