TY  - JOUR
TT  - Mass Spectrometric Quantitation of Serum Cephazolin
AU  - Unlu, Ali
AU  - Abusoglu, Sedat
AU  - Sivrikaya, Abdullah
PY  - 2017
DA  - November
JF  - The Turkish Journal Of Occupational / Environmental Medicine and Safety
JO  - turjoem
PB  - Engin TUTKUN
WT  - DergiPark
SN  - 2149-4711
SP  - 61
EP  - 61
VL  - 2
IS  - 1(4)
KW  - Mass Spectrometric Quantitation of Serum Cephazolin
N2  - ObjectivesCefazolin, a first generationcephalosporin β-lactam antibiotic, is commonly used forprophylaxis during cesarean delivery, and in treatment of pulmonary infectionsand many surgical procedures. The aim of this work was to develop a simple,fast and accurate liquid-chromatography-mass spectrometry method fordetermination of serum cefazolin. MethodsMass spectrometric analyses wereperformed using an Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API3200 triple quadrupole mass spectrometer (USA) equipped with an electrosprayion source (ESI) operating in positive mode. ResultsThe standard curves for cefazolin waslinear within the range of 0.39-200 µg/ml. Total run time was 5 minutes.Chromatographic separation was performed on a C18 column (4.6×50 mm, 5 µm, Phenomenex Luna) with a mobile phase consisting of 1%formic acid in water and MeOH (10:90, V/V)at a flow rate of 0.8 ml/min.  DiscussionSerum cefazolin measurement can beeasily performed by LC-MS/MS system to identify the risk of the patients withtherapeutic drug monitoring. This method performs this drug analysis with highthroughput. ConclusionsExact determination of cefazolinprovides reliable levels in clinical studies to avoid toxic effects. KeywordsAntibiotic levels, mass spectrometry,cefazolin, toxicity. Acknowledgments: No conflict ofinterest
CR  - Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
CR  - Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey
UR  - https://dergipark.org.tr/tr/pub/turjoem/issue//386041
L1  - https://dergipark.org.tr/tr/download/article-file/413947
ER  -