TY  - JOUR
TT  - In vitro effects of ethanol and aspirin combined administration On erythrocyte membrane properties
AU  - Kanbak, Güngör
AU  - Çalışkan, Figen
AU  - Özdemir, Filiz
AU  - İnal, Mine
PY  - 2009
DA  - April
JF  - Pamukkale Medical Journal
JO  - Pam Tıp Derg
PB  - Pamukkale Üniversitesi
WT  - DergiPark
SN  - 1308-0865
SP  - 1
EP  - 7
IS  - 1
KW  - Asetil salisilik asit
KW  - s-adenozilmetiyonin
KW  - etanol
KW  - Na+K+ ATPaz
KW  - Sialik Asit
KW  - MDA
N2  - This report describes in vitro effect of ethanol and combined administration of ethanol with aspirin on erythrocyte membrane properties. Furthermore, we evaluated the possible protective role of sAdenosylmethionine (SAMe) and for this aim, we determined plasma malondialdehyde (MDA), sialic acid (SA) levels and the activity of membrane-bound Na+ -K+ATPase. Concentration doses were adjusted as control (0% ethanol), ethanol (0,5 %), aspirin (4 mg/ml) and SAMe (2.5 nM). Blood samples were divided into five groups as control, ethanol, [ethanol+aspirin], [ethanol+SAMe] and [ethanol+SAMe] group and incubated on 25°C for 16 h. After this, we measured Na+ -K+  ATPaz, MDA, Sialik Asit levels. Na+ -K+ATPase activities were decreased in the ethanol and 0,5% [ethanol+SAMe] groups as compared to the control group (p<0.05). Plasma MDA levels in the [ethanol+aspirin] and [ethanol+aspirin+SAMe] groups were higher than in the control group (p<0.001), also the [ethanol+aspirin] group was higher than in the ethanol group (p<0.001). SA levels in the [ethanol+SAMe] groups were increased significantly as compared to the control group (p<0.01). We assume that aspirin protected ethanol-induced inactivation of Na+ -K+ ATPase activities and also decreased sialic acid levels. But, combined administration of aspirin with ethanol increased peroxidative injury of erythrocyte membranes. We think that protective role of SAMe may be dosedependent.
UR  - https://dergipark.org.tr/tr/pub/patd/issue//392563
L1  - https://dergipark.org.tr/tr/download/article-file/422274
ER  -