TY  - JOUR
TT  - The investigation of the effects of Crataegus aronia var. dentata Browicz extract on spleen:  a histochemical study
AU  - Keskin, Nazan
AU  - Mammadov, Ramazan
AU  - İli, Pınar
PY  - 2012
DA  - July
JF  - Pamukkale Medical Journal
JO  - Pam Tıp Derg
PB  - Pamukkale Üniversitesi
WT  - DergiPark
SN  - 1308-0865
SP  - 68
EP  - 74
IS  - 2
KW  - Crataegus aronia var. dentata Browicz
KW  - dalak
KW  - histokimya
N2  - OBJECTIVE:  Spleen is an organ of immune and hematopoietic system and involves extensive cellular interactions. Cell surface and extracellular matrix carbohydrates are important in cellular interactions (cell-cell and cell-matrix). Spleen is affected by the exposure to toxic agents. Crataegus (Hawthorn) species, which contains mainly flavonoid compounds, have such effects as antioxidant, cardioprotective, antiinflammatory and anticarcinogenic.In this study, the effects of Crataegus aronia var. dentata Browicz extracts on spleen were investigated via light microscopy.METHODS:  The plant extract at concentration of 1% was given orally to rats for 6 weeks. At theend of the experimental period, tissues were fixed, processed and embedded in paraffin. 5 μm sections were stained with Hematoxylin and Eosin, Periodic acid-Schiff stain for histomorphology and Perl’s Prussian blue stain for hemosiderin. For lectin histochemistry, frozen sections were treated with three digoxigenin labelled lectins; peanut agglutinin (PNA) specific for galactose (1→3) N-acetylgalactosamine [Galβ(1→3)GalNAc];Maackia amurensis leucoagglutinin (MAL) specific for sialic acidα(2→3)Galactose; Galanthus nivalis agglutinin (GNA) specific for terminal mannose residues.RESULTS:  In general, the histomorphology of the spleens and the accumulation of hemosiderin in macrophages were similar for both control and experimental groups. Lectins showed positive reactions in some cell cytoplasms and on the cell surfaces of both groups. A little more intensive GNA-positive reactions were observed on thecell surfaces.CONCLUSION:  The plant extract at concentration of 1% did not lead to any alterations in some carbohydrate molecules and hemosiderin in the spleen.
UR  - https://dergipark.org.tr/tr/pub/patd/article/392747
L1  - https://dergipark.org.tr/tr/download/article-file/422475
ER  -