TY  - JOUR
T1  - In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.
AU  - Çetin, Burcu
AU  - Akın, Betül
AU  - Akanıl Bingöl, Nüket
PY  - 2018
DA  - December
DO  - 10.18466/cbayarfbe.393626
JF  - Celal Bayar University Journal of Science
JO  - CBUJOS
PB  - Manisa Celal Bayar Üniversitesi
WT  - DergiPark
SN  - 1305-130X
SP  - 369
EP  - 372
VL  - 14
IS  - 4
LA  - en
AB  - The aim of this experiment is to develop a protocol to establish the in vitro propagation potential of Lythrum salicaria L (purple loosestrife).Seeds of L. salicaria were germinatedand grown in Murashige Skoog (MS) medium for 30 days. At the end of 30 days, shoot tips of L. salicaria were cultured on MS medium supplemented with differentcombinations and concentrations of 6-benzylaminopurine (BAP; 1, 2 and 3 mg/L) and1-naphthaleneacetic acid (NAA; 0, 0.1 and 0.5 mg/L). Regenerated shoots were transferredto MS supplemented with varying concentrations of different auxins: NAA (0.5and 1 mg/L) and indole-3-butyric acid (IBA; 0.5 and 1.0 mg/L) for rooting. Amongthe cytokinins investigated, BAP indicated the best response to initiate shootelongation and multiple shoot formation alone. The highest number of shoot perexplant (37±3.38) was obtained in MS medium with 1 mg/L BAP. Although, thehighest shoot length (3.1 cm ± 0.48) was found on medium with 1 mg/L BAP+0.5 mg/LNAA, there were no statistically differences among all types of medium.Percentage of rooting, shoot length and root length were altered significantlywith different concentrations of NAA and IBA. Thus, shoot length (9.5 cm±0.46),root length (4.1 cm±0.13) and the percentage of rooting (100%) increasedsignificantly with a concentration of 1 mg/L NAA. It was concluded that MS medium with 1 mg/LNAA has been superior for root development compared to other hormoneconcentrations.
KW  - auxins
KW  - cytokinins
KW  - purple loosestrife
KW  - rooting
KW  - tissue culture
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UR  - https://doi.org/10.18466/cbayarfbe.393626
L1  - https://dergipark.org.tr/tr/download/article-file/606193
ER  -