@article{article_394913, title={Determination of Amitriptyline and Nortriptyline in Biological Fluids by Thin-Layer and Gas Chromatography}, journal={Journal of Faculty of Pharmacy of Ankara University}, volume={10}, pages={69–79}, year={1980}, DOI={10.1501/Eczfak_0000000169}, url={https://izlik.org/JA57YK74FC}, author={Vural, Nevin and Söylemezoğlu, Tülin and Güvendik, Gülin}, abstract={Thin-Layer Chromatagraphy was used as a serening test in this research for urine and plazma extracts containing amitripthyline and nortriptyline at concentrations 5,25, 50 and 100 g / 5 ml biologic fluid. Extraction was made with n- heptane at pH 11. The developing system (ethyl ecetate: n-heptane: methyl alcohol:ammonia: 60:30: 7-3:7-5) we used on Silicagel-G plates gave the test separation and 1 g drug can be detected using Dragendorff reagent. Gas-Liquid Chromatography (GLC) was used for confirmation and for the quantitative determination of the drugs in the extract. Analyses were performed on Packard-Becker 419 Gas Chromatograph equipped with FID and glass column 2 m X 4 mm (i.d) packed with 3% OV-17 on Chromosorb W-HP, 100-120 mesh and protriptyline was used as an internal standart. The lower limit of sensitivity with GLC was 0.5 g/ml biologic fluid for amitriptyline and 1 g/ml biologic fluid for nortriptyline. The receveries were 62 1.3 % for amitriptyline and 74.3 1.5 % for nortriptyline.}, number={1}