TY - JOUR T1 - Antimicrobial Activity of Phytosphingosine Nanoemulsions against Bacteria and Yeasts AU - Başpınar, Yücel AU - Kotmakçı, Mustafa AU - Öztürk, İsmail PY - 2018 DA - June DO - 10.18466/cbayarfbe.403152 JF - Celal Bayar University Journal of Science JO - CBUJOS PB - Manisa Celal Bayar Üniversitesi WT - DergiPark SN - 1305-130X SP - 223 EP - 228 VL - 14 IS - 2 LA - en AB - Phytosphingosine(PS) is a natural antimicrobial ingredient present in the mammalian stratumcorneum of the skin. The skin surface is the first line defense system againstmicrobial colonization and infections. The aim of this study was to investigatein vitro antimicrobial effects of PSand PS-hydrochloride (PSHCl) nanoemulsion (NE) formulations and solutionsagainst bacteria and fungi. Minimum inhibitory concentrations (MICs) of thePS-NE and PSHCl-NE formulations and PS/PSHCl solutions were determined bymicrodilution method against Gram-positive/Gram-negative bacteria and yeasts.Inhibitory effects were also examined by disc diffusion method and inhibitionzone diameters were determined. PS/PSHCl solutions and PS-NE/PSHCl-NEformulations were prepared in different conditions. Physicochemical propertiesof the NEs were characterized and antimicrobial activities were evaluated. Inthis study we reported that the PS and PSHCl and NEs had variable antimicrobialactivities for a variety of Gram-positive bacteria (MIC range 1 µg/ml to 1024µg/ml), Gram-negative bacteria (MIC range 8 µg/ml to 1024 µg/ml) and Candidastrains (MIC range 8 µg/ml to 512 µg/ml). Sphingolipids are part of the naturaldefense system of the body. We demonstrated that PS and PSHCl solutions and NEshave broad antimicrobial activity against bacteria and yeasts. In addition, thenewly developed formulations may have potential for prophylactic andtherapeutic intervention in infections. KW - Phytosphingosine KW - Phytosphingosine hydrochloride KW - Nanoemulsions KW - Antimicrobial activity CR - 1. Wolf, F, Juestel, C, Schreiber, J, Klier, M, Sphingolipids as antimicrobial agents, German Patent 1997, 19602108. 2. Lersch, P, Schick, U, Choosing the right ingredients for cosmeceuticals, Speciality Chemicals Magazine, 2003, 23, 30-31. 3. Gupta, A.K, Fisher, G.J, Elder, J.T, Nickoloff, B.J, Voorhees, J.J, Sphingosine inhibits phorbol esterinduced inflammation, ornithine decarboxylase activity, and activation of protein kinase C in mouse skin. Journal of Investigative Dermatology, 1988, 91, 486–491. CR - 4. Rawlings, A.V, 2003. Trends in stratum corneum research and the management of dry skin conditions, International Journal of Cosmetic Science, 53, 63–95. CR - 5. Drake, D.R, Brogden, K.A, Dawson, D.V, Wertz, P.W, Thematic review series: skin lipids. Antimicrobial lipids at the skin surface. Journal of Lipid Research, 2008, 49, 4 –11. CR - 6. Mason, T.G, Wilking, J.N, Meleson, K, Chang, C.B, Graves, S.M, Nanoemulsions: Formation, structure and physical properties. Journal of Physics: Condensed Matter, 2006, 18, 635–666. CR - 7. Bibel, D.J, Aly, R, Shinefield, H.R, Antimicrobial activity of spingosines. Journal of Investigative Dermatology, 1992, 98, 269– 273. CR - 8. Fischer, C.L, Drake D.R, Dawson D.V, Blanchette, D.R, Brogden, K.A, Wertz P.W, Antibacterial Activity of Sphingoid Bases and Fatty Acids against GramPositive and Gram-Negative Bacteria, Antimicrobial Agents and Chemotherapy, 2012, 56(3), 1157-1161. CR - 9. Baspinar, Y, Keck, C, Borchert, H.H, Development of a positively charged prednicarbate nanoemulsion, International Journal of Pharmaceutics, 2010, 383, 201–208. CR - 10. Baspinar, Y, and Borchert, H.H, Penetration and release studies of positively and negatively charged nanoemulsions—Is there a benefit of the positive charge? International Journal of Pharmaceutics, 2012, 430, 247-252. CR - 11. Kotmakçı, M, Öztürk, İ, Kantarcı, G, Ermertcan, Ş. Characterization and antimicrobial activity of novel hazelnut oil microemulsion loaded with Mitomycin C. Latin American Journal of Pharmacy, 2015, 34: 529– 36. CR - 12. Fischer, C.L, Walters, K.S, Drake, D.R, Blanchette, D.R, Dawson, D.V, Brogden, K.A, Wertz, P.W, Sphingoid bases are taken up by Escherischia coli and Staphylococcus aureus and induce ultrastructural damage, Skin Pharmacology and Physiology, 2013, 26, 36–44. CR - 13. Fischer, C.L, Blanchette, D.R, Brogden, K.A, Dawson, D.V, Drake, D.R, Hill, J.R, Wertz, P.W, The roles of cutaneous lipids in host defense, Biochimica et Biophysica Acta, 2014, 1841(3), 319322. CR - 14. Drake, D.R, Brogden, K.A, Dawson, D.V, Wertz, P.W, Thematic review series: skin lipids. Antimicrobial lipids at the skin surface. Journal of Lipid Research, 2008, 49, 4–11. CR - 15. Pavicic, T, Wollenweber, U, Farwick, M, Korting, H.C, Anti-microbial and -inflammatory activity and efficacy of phytosphingosine: an in vitro and in vivo study addressing acne vulgaris. International Journal of Cosmetic Science, 2007, 29, 181–190. CR - 16. Hoeller, S, Sperger, A, Valenta, C, Lecithin based nanoemulsions: a comparative study of the influence of non-ionic surfactants and the cationic phytosphingosine on physicochemical behaviour and skin permeation. International Journal of Pharmaceutics, 2009, 370, 181–186. CR - 17. Hasanovic, A, Hoeller, S, Valenta, C, Analysis of skin penetration of phytosphingosine by fluorescence detection and influence of the thermotropic behaviour of DPPC liposomes, International Journal of Pharmaceutics, 2010, 4, 383(1-2), 14-17. CR - 18. Bergsson, G, Arnfinnsson, J, Steingrimsson, O, Thormar, H, In vitro killing of Candida albicans by fatty acids and monoglycerides, Antimicrobial Agents and Chemotherapy, 2001, 45, 3209 –3212. CR - 19. Veerman, E.C, Valentijn-Benz, M, van't Hof, W, Nazmi, K, van Marle, J, Amerongen, A.V, Phytosphingosine kills Candida albicans by disrupting its cell membrane, Biological Chemistry, 2010, 391(1), 65-71. CR - 20. Garg, S.K, Volpe, E, Palmiero, G, Mattei, M, Galati, D, Martino, A, Piccioni, M.S, Valente, E, Bonanno, E, De Vito, P, Baldini, P.M, Spagnoli, L.G, Cilizzi, V, Fraziano, M, Sphingosine 1-phosphate induces antimicrobial activity both in vitro and in vivo. Journal of Infectious Diseases, 2004, 189, 2129–2138. UR - https://doi.org/10.18466/cbayarfbe.403152 L1 - https://dergipark.org.tr/tr/download/article-file/497167 ER -