TY  - JOUR
T1  - TOKSİKOLOJİK ARAŞTIRMALARDA ALTERNATİF BİR ORGANİZMA:  Caenorhabditis elegans  (C. elegans)
TT  - AN ALTERNATIVE ORGANISM IN TOXICOLOGICAL RESEARCHES: Caenorhabditis elegans  (C. elegans)
AU  - Öğüt, Serdal
AU  - Savaş, Nazmi
AU  - Olgun, Abdullah
PY  - 2018
DA  - May
JF  - Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi
PB  - Aydın Adnan Menderes  Üniversitesi
WT  - DergiPark
SN  - 2587-2427
SP  - 99
EP  - 106
VL  - 2
IS  - 2
LA  - tr
AB  - Sonyıllarda toksik ve toksik olma potansiyeli olan maddeler artış göstermektedir. Butoksik maddeler çevre ve halk sağlığını olumsuz etkilemektedir. Bu olumsuzetkileri göstermek amacı ile birçok model organizma kullanılmaktadır. Caenorhabditis elegans (C. elegans) toksisite çalışmaları içinkullanılan önemli model organizmalardan biridir. Bu organizmanın birçokkullanım amacı mevcuttur. Diğer model organizmalar ile karşılaştırıldığındatoksikoloji çalışmalarında kullanılmasının bazı avantajları ve dezavantajlarıvardır. En önemli avantajlarından birisi de otomatik yüksek ölçekli taramalarınyapılabilmesi sayesinde aynı anda yüzlerce hatta binlerce maddenin toksisitetaramasının yapılabilmesidir. Bu derlemede C.elegans hakkında ve toksisite araştırmalarındaki kullanımı hakkındabilgiler verilmiştir.
KW  - Caenorhabditis elegans
KW  - model organizma
KW  - toksikoloji
N2  - Inrecent years, toxic and potentially toxic substances have increased. Thesetoxic substances affect the human health and environment in a negative way. Ithas been used many model organisms to show the negative effects. Caenorhabditis elegans (C. elegans) isone of the most important model organisms used for the toxicity studies. Thisorganism has lots of intended purposes. When compared to the other organisms,it has some advantages and disadvantages on the use of toxicology studies. Oneof the most important advantages is that thanks to the automatic high-scalescans, it can be performed to scan for toxicity of hundreds or even thousandsof substances at the same time. In this compilation, informations have beenprovided about C. elegans and its usein toxicity studies.
CR  - KAYNAKLAR1- Ünlü E.  Deri yaşlanmasında korunma ve tedavi yöntemleri. Dermatoz 1, 2010; (1): 23-31.2-Brenner S.  The genetics of C. elegans. Genetics, 1973; (77): 71-94.3- Riddle DL, Blumenthal T, Meyer JB, Priess JR, ‘’ C. elegans II, 2nd edition’’, Cold Spring Harbor Mongraph Series, 2010; 33.4- Horvitz HR. A nematode as a model organism: the genetics of programmed death [Film]. Cogito Learning Media, Inc. Available: http://www.cogitomedia.com [1999, Jul 20].5- www.wormatlas.org 6- www.wormclassroom.org7- Hunt PR. The C. elegans model in toxicity testing. J. Appl. Toxicol., 2017; 37: 50–59.8- Nass R, Hamza I. The nematode C. elegans as an animal model to explore toxicology in vivo: solid and axenic growth culture conditions and compound exposure parameters. Curr. Protoc. Toxicol., Chapter 1: 2007; Unit1 9, doi: 10.1002/0471140856.tx0109s31.
CR  - 9- Tralau T, Riebeling C, Pirow R, Oelgeschlager M, Seiler A, Liebsch M, Luch A. Wind of change challenges toxicological regulators. Environ. Health Perspect. 2012; 120: 1489–1494.
CR  - 10- Hartung T. Toxicology for the twenty-first century. Nature, 2009; 460: 208–212.
CR  - 11- Knight AW, Little S, Houck K, Dix D, Judson R, Richard A, McCarroll N, Akerman G, Yang C, Birrell L, Walmsley RM. Evaluation of highthroughput genotoxicity assays used in profiling the US EPA ToxCast chemicals. Regul. Toxicol. Pharmacol., 2009; 55: 188–199.
CR  - 12- Miranda JP, Leite SB, Muller-Vieira U, Rodrigues A, Carrondo MJ, Alves PM. Towards an extended functional hepatocyte in vitro culture. Tissue Eng. Part C Methods, 2009; 15: 157–167.
CR  - 13- Scott CW, Peters MF, Dragan YP. Human induced pluripotent stem cells and their use in drug discovery for toxicity testing. Toxicol. Lett., 2013; 219: 49–58.
CR  - 14- Tice RR, Austin CP, Kavlock RJ, Bucher JR. Improving the human hazard characterization of chemicals: a Tox21 update. Environ. Health Perspect, 2013; 121: 756–765.
CR  - 15- Casey W, Jacobs A, Maull E, Matheson J, Clarke C, Lowit A. A new path forward: the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and National Toxicology Program’s Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). J. Am. Assoc. Lab. Anim. Sci., 2015; 54: 170–173.
CR  - 16- NTP. West Virginia Chemical Spill: NTP Studies and Results. National Toxicology Program. http://ntp.niehs.nih.gov/results/areas/wvspill/studies/ index.html [accessed 4 June 2015].
CR  - 17- Dong Zhou, Jie Yang, Hui Li , Changzheng Cui, Yunjiang Yu, Yongdi Liu, Kuangfei Lin. The chronic toxicity of bisphenol A to Caenorhabditis elegans after long-term exposure at environmentally relevant concentrations. Chemosphere, 2016; 154, 546-551.
CR  - 18- Li Y, Gao S, Jing H, Qi L, Ning J, Tan Z, Yang K, Zhao C, Ma L, Li G. Correlation of chemical acute toxicity between the nematode and the rodent. Toxicol. Res., 2013; 2: 403–412.
CR  - 19- Cole RD, Anderson GL, Williams PL. The nematode Caenorhabditis elegans as a model oforganophosphate-induced mammalian neurotoxicity. Toxicol. Appl. Pharmacol., 2004; 194: 248.
CR  - 20- Pirinç B, Türkoğlu Ş.  Etil Paraben ve Metil Parabenin Caenorhabditis Elegans’ta Yumurta Verimi, Yaşama Yüzdesi ve Fiziksel Büyüme Üzerine Olan Etkilerinin Araştırılması. Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi (CFD), 2016; Cilt 37, No. 4.
CR  - 21- Moy TI, Conery AL, Larkins-Ford J, Wu G, Mazitschek R, Casadei G, Lewis K, Carpenter AE, Ausubel FM. High-throughput screen for novel antimicrobials using a whole animal infection model. ACS Chem. Biol., 2009; 4: 527–533.
CR  - 22- Lakshmanan U, Yap A, Fulwood J, Yichun L, Hoon SS, Lim J, Ting A, Sem XH, Kreisberg JF, Tan P, Tan G, Flotow H. Establishment of a novel whole animal HTS technology platform for melioidosis drug discovery. Comb. Chem. High Throughput Screen, 2014; 17: 790–803.
CR  - 23- Stutz K, Kaech A, Aebi M, Kunzler M, Hengartner MO. Disruption of the C. elegans Intestinal Brush Border by the Fungal Lectin CCL2 Phenocopies Dietary Lectin Toxicity in Mammals. PLoS One, 2015; 10: e0129381.
CR  - 24- Maxwell C.K. Leung, Phillip L. Williams,  Alexandre Benedetto, Catherine Au,‡ Kirsten J. Helmcke, Michael Aschner, and Joel N. Meyer. Caenorhabditis elegans: An Emerging Model in Biomedical and Environmental Toxicology. Toxicological Sciences, 2008; 106(1), 5–28.
CR  - 25- Anderson GL, Cole RD, Williams PL.  Assessing behavioral toxicity with Caenorhabditis elegans. Environ. Toxicol. Chem., 2004; 23: 1235–1240.
CR  - 26- Bergin IL, Wilding LA, Morishita M, Walacavage K, Ault AP,Axson JL, Stark DI, Hashway SA, Capracotta SS, Leroueil PR, Maynard AD, Philbert MA. Effects of particle size and coating on toxicologic parameters, fecal elimination kinetics and tissue distribution of acutely ingested silver nanoparticles in a mouse model. Nanotoxicology, 2016; 10: 352-360.
CR  - 27- Boyd WA, McBride SJ, Rice JR, Snyder DW, Freedman JH. A highthroughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay. Toxicol. Appl. Pharmacol. 2010; 245: 153–159.
CR  - 28- Dhawan R, Dusenbery DB, Williams PL. Comparison of lethality, reproduction, and behavior as toxicological endpoints in the nematode Caenorhabditis elegans. J. Toxicol. Environ. Health A, 1999; 58: 451–462.
CR  - 29- Hunt PR, Olejnik N, Sprando RL. Toxicity ranking of heavy metals with screening method using adult Caenorhabditis elegans and propidium iodidereplicatestoxicityrankinginrat. Food Chem. Toxicol., 2012; 50: 3280–3290.
CR  - 30- Leung MCK, Williams PL, Benedetto A, Au C, Helmcke KJ, Aschner M, Meyer JN.  Caenorhabditis elegans: an emerging model in biomedical and environmental toxicology. Toxicol. Sci., 2008; 106: 5 –28.
CR  - 31- Boyd WA, McBride SJ, Rice JR, Snyder DW, Freedman JH. A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction essay. Toxicol. Appl. Pharmacol., 2010; 245: 153-159.
CR  - 32-  Cornaglia M, Lehnert T, Gijs MAM. Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans. Roy. Soc. Chem., 2017; DOI: 10.1039/C7LC00509A.
UR  - https://dergipark.org.tr/tr/pub/amusbfd/issue//412510
L1  - https://dergipark.org.tr/tr/download/article-file/517718
ER  -