TY  - JOUR
T1  - Adsorption of DNA on Coated Magnetic Beads: Comparison of Physical and Chemical Adsorption
TT  - Kaplanmış Manyetik Kürelere DNA Adsorpsiyonu: Fiziksel ve Kimyasal Adsorpsiyonun Karşılaştırılması
AU  - Bayraç, Ceren
AU  - Bayraç, Abdullah Tahir
AU  - Sarıkaya, Ecem
AU  - Varçin, Merve
PY  - 2019
DA  - March
DO  - 10.18185/erzifbed.442557
JF  - Erzincan University Journal of Science and Technology
PB  - Erzincan Binali Yıldırım Üniversitesi
WT  - DergiPark
SN  - 2149-4584
SP  - 215
EP  - 224
VL  - 12
IS  - 1
LA  - en
AB  - Based on their unique propertiesvarious iron magnetic nanoparticles have proved to be excellent nanomaterialsfor applications in separation and concentration process. Immobilization ofdeoxyribonucleic acid (DNA) molecules on the magnetic beads are acutelyimportant and have potential uses in many techniques such as DNA extraction,concentration, biosensors, microarrays and next generation sequencing. In thisstudy the adsorption of single stranded DNA (ssDNA) via poly-l-lysine coatediron oxide magnetic beads was performed under varying conditions ofpoly-l-lysine amount, initial DNA concentration, ionic strength, bonding typeand length of DNA. The adsorption process was examined via Langmuir andFreundlich isotherm models. The ionic interaction between negatively chargedDNA and positively charged surface of magnetic beads showed multilayeradsorption with Freundlich adsorption isotherm, covalent bonding betweenmodified DNA and surface by crosslinking provided higher adsorption efficiencywith Langmuir adsorption isotherm. Both adsorption methods provided magneticbeads with favorable adsorption of ssDNA.
KW  - ssDNA
KW  - magnetic beads
KW  - adsorption
KW  - Langmuir
KW  - Freundlich
N2  - Kendilerine hasözellikleri nedeniyle çeşitli demir manyetik nanopartiküllerin ayırma ve yoğunlaştırmauygulamalarında mükemmel nanomateryal oldukları kanıtlanmıştır.Deoksiribonükleik asit (DNA) moleküllerinin manyetik kürelere immobilizasyonuson derece önemli olmakla birlikte DNA ekstraksiyonu, yoğunlaştırılması,biyosensörler, mikrodiziler ve yeni nesil dizileme gibi birçok tekniktepotansiyel kullanım alanına sahiptir. Bu çalışmada, poli-l-lizin miktarı,başlangıç ​​DNA konsantrasyonu, iyonik kuvveti, bağlanma tipi ve DNAuzunluğunun değişen koşulları altında poli-l-lizin kaplı demir oksit manyetikküreler aracılığıyla tek iplik DNA'nın (ssDNA) adsorpsiyonu,gerçekleştirilmiştir. Adsorpsiyon işlemi Langmuir ve Freundlich izotermmodelleri ile incelenmiştir. Negatif yüklü DNA ile pozitif yüklü manyetikküreler arasındaki iyonik etkileşimi Freundlich adsorpsiyon izotermiyle çokkatmanlı adsorpsiyon, modeli gösterirken, DNA ve yüzey arasındaki kovalentbağlanma Langmuir adsorpsiyon izoterminde daha yüksek adsorpsiyon verimigöstermiştir. Her iki adsorpsiyon yöntemi, manyetik kürelere uygun ssDNAadsorpsiyonu sağlamıştır.
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UR  - https://doi.org/10.18185/erzifbed.442557
L1  - http://dergipark.org.tr/tr/download/article-file/677623
ER  -