TY  - JOUR
T1  - Mercimek (Lens culinaris M.) DEHYDRATION RESPONSIVE ELEMENTBINDING2A (DREB2A) Geninin Kuraklık Stresi Koşullarındaki İfadesinin Belirlenmesi
TT  - Determination of Lentil (Lens culinaris M.) DEHYDRATION RESPONSIVE ELEMENT-BINDING2A (DREB2A) Gene Expression under Drought Stress Conditions
AU  - Bakır, Melike
PY  - 2019
DA  - June
Y2  - 2018
DO  - 10.20289/zfdergi.448332
JF  - Journal of Agriculture Faculty of Ege University
PB  - Ege Üniversitesi
WT  - DergiPark
SN  - 1018-8851
SP  - 181
EP  - 185
VL  - 56
IS  - 2
LA  - tr
AB  - Amaç: Dehydration responsive element binding (DREB) proteinleri, bitkilerde strese yanıt ve sinyaliletiminde önemli rol oynarlar. Bu çalışmada, DREB gen ailesinin üyesi olan ve LcDREB2A olarakisimlendirilen mercimek (Lens culinaris M.) DREB2A geninin kısmi cDNA’sı izole edilmiş ve kuraklıkstresi ile ilişkisi belirlenmiştir.Materyal ve Metot: Kuraklık stresi ve LcDREB2A gen ifadesi arasındaki ilişkiyi anlamak için, 2 haftasüre ile yetiştirilen mercimek fidelerine 6, 13 ve 20 gün süre ile sulamama şeklinde kuraklık stresiuygulanmıştır. Kök ve yapraklarda meydana gelen gen ifadesi değişimleri eş zamanlı kantitatif PCR(Real-time qPCR) ile belirlenmiştir.Bulgular: LcDREB2A gen ifadesi, Fırat87 ve Özbek çeşidinin yapraklarında artan kuraklık stresi ileartmış ve kuraklığın 20. gününde en yüksek seviyeye ulaşmıştır. Diğer taraftan Özbek çeşidininköklerinde gen ifadesi seviyesi 6. günde en yüksek seviyeye ulaşırken 13. günde azalmış ve 20.günde tekrar artış göstermiştir. Fırat87 çeşidinde ise, artan kuraklık stresi ile birlikte düzenli bir artışgörülmüş ve 20. günde en yüksek seviyeye ulaşmıştır.Sonuç: LcDREB2A geninde gözlenen doku spesifik gen ifadesi profili, bu transkripsiyon faktörününmercimek bitkisinde kuraklık stresindeki rolünü ve kompleks regülasyonunu göstermektedir.
KW  - Mercimek
KW  - DREB2A
KW  - kuraklık
KW  - gen ifadesi
KW  - eş zamanlı kantitatif PCR
N2  - Objective: Dehydration responsive element binding (DREB) proteins are important transcriptionfactors in plant stress response and signal transduction. In this study, partial cDNA of lentil (Lensculinaris M.) DREB2A, namely LcDREB2A, which belongs to the DREB gene family was isolated andidentified its relationship with drought stress.Material and Methods: Two-week-old plants were subjected to drought stress through irrigationsfor 6, 13 and 20 days to elucidate the relationship between the expression profile of LcDREB2Agene and drought stress. Changes of gene expression in lentil roots and leaves were assayed byquantitative real time PCR.Results: The expression of LcDREB2A in both Fırat87 and Özbek lentil leaves increased withdecreasing water contents and reached to a maximum on the 20th day of dehydration. On the otherhand, in Özbek roots, the highest level of expression was observed on the 6th day of dehydration.The levels decreased on 13th day and increased on 20th day. Level of expression in Fırat87 rootsdecreased with increasing water contents and the greatest level of expression was observed onthe 20th day of dehydration.Conclusion: Observed tissue-specific expression profile of LcDREB2A suggested a complexregulation and indicated the role of this transcription factor in lentil drought response.
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UR  - https://doi.org/10.20289/zfdergi.448332
L1  - https://dergipark.org.tr/tr/download/article-file/748359
ER  -