TY  - JOUR
T1  - Antibacterial Activity of  DMSO Extracts of Selected Plants Against Antibiotic Resistant Clinical Isolates
TT  - Seçilen Bitkilerin DMSO Özütlerinin Antibiyotik Dirençli Klinik İzolatlara Karşı Antibakteriyel Aktivitesi
AU  - Saral, Ayşegül
AU  - Kardil, Uğur
AU  - Özad Düzgün, Azer
PY  - 2019
DA  - August
DO  - 10.18185/erzifbed.451871
JF  - Erzincan University Journal of Science and Technology
PB  - Erzincan Binali Yıldırım Üniversitesi
WT  - DergiPark
SN  - 2149-4584
SP  - 576
EP  - 584
VL  - 12
IS  - 2
LA  - en
AB  - In this study, we aimed to find out new herbal materialsthat are able to inhibit the growth of the P.aeruginosa and E.coli clinical isolatesthat has antibiotic resistance. Clinical isolates used in this research are E. coli (n=1) and P.aeruginosa (n=1). Antibiotic susceptibility profiles of E. coli and P. aeruginosa were determined using e-test. Plants were collectedin Trabzon region of Turkey are Calendula officinalis, Hypericum perforatum and Glycyrrhiza glabra. DMSO were used assolvent and solid-liquid extraction was employed. Micro-dilution method waspreferred fo the determination of the minimum inhibitory concentration (MIC). MICresults were obtained through observation of turbidities. According to E-testresults, while P. aeruginosa wasresistant to piperacillin, piperacillin/tazobactam, meropenem and ceftazidime, E. coli was resistant to piperacillin,cefotaxime and ceftazidime. DMSO extract of Calendulaofficinalis showed very strong activity against PA1 with the best MIC (5mg/mL). DMSO extract of three plant hadlower MIC values (5-10 mg/ml) for EC1 and PA1 than ampicillin. In futurestudies antibacterial activity of different solvents extracts of these plantsand other plants against antibiotic resistant clinical isolates will beexamined. Natural products from plants are promising in fighting withantibiotic-resistant bacteria.
KW  - Antibiotic Resistance
KW  - Pseudomonas aeruginosa
KW  - Escherichia coli
KW  - Minimum Inhibitory Concentration
KW  - Plant Extract
N2  - Bu çalışmada, antibiyotik dirençli P. aeruginosa ve E.coliklinik izolatlarının büyümesini engelleyebilecek yeni bitkisel materyallerinkeşfi amaçlanmıştır. Bu çalışmada kullanılan klinik izolatlar E. coli (n = 1) ve P.aeruginosa'dır (n = 1). E.coli ve P. aeruginosa'nınantibiyotik duyarlılık profilleri e-test kullanılarak belirlenmiştir. Trabzonyöresinden Calendula officinalis,Hypericum perforatum and Glycyrrhizaglabra bitkileri toplanmıştır. Çözücü olarak DMSO kullanılmış ve katı-sıvıekstraksiyon yapılmıştır. Minimum inhibitör konsantrasyonunun (MİK)belirlenmesi için mikro dilüsyon yöntemi tercih edilmiştir. MİK sonuçlarıtürbiditelerin gözlemlenmesiyle elde edilmiştir. E-test sonuçlarına göre, P. aeruginosa piperasilin,piperasilin/tazobaktam, meropenem ve seftazidime dirençli iken, E. coli piperasilin, sefotaksim veseftazidime dirençliydi. Calendulaofficinalis'in DMSO özütü, en iyi MIC (5 mg / mL) ile PA1'e karşı çok güçlüaktivite göstermiştir. Üç bitkinin DMSO özütü, EC1 ve PA1 için ampisilindeğerinden daha düşük MIC değerlerine (5-10 mg / ml) sahip olduğu tespitedilmiştir. Gelecekteki çalışmalarda, bu bitkilerin ve diğer bitkilerinantibiyotik dirençli klinik izolatlara karşı farklı çözücülerle hazırlananözütlerinin antibakteriyel aktiviteleri incelenecektir. Bitkilerden elde edilendoğal ürünler, antibiyotiğe dirençli bakterilerle savaşırken ümit vericidir.
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UR  - https://doi.org/10.18185/erzifbed.451871
L1  - https://dergipark.org.tr/tr/download/article-file/797492
ER  -