TY  - JOUR
T1  - Biochemical and molecular tolerance of Carpobrotus acinaciformis L. halophyte plants exposed to high level of NaCl stress
TT  - NaCl stresine maruz bırakılan Carpobrotus acinaciformis L. halofit bitkisinin biyokimyasal ve moleküler tepkileri
AU  - Karakaş Dikilitaş, Sema
AU  - Dikilitaş, Murat
AU  - Tıpırdamaz, Rukiye
PY  - 2019
DA  - March
DO  - 10.29050/harranziraat.464133
JF  - Harran Tarım ve Gıda Bilimleri Dergisi
PB  - Harran Üniversitesi
WT  - DergiPark
SN  - 2587-1358
SP  - 99
EP  - 107
VL  - 23
IS  - 1
LA  - tr
AB  - Carpobrotus acinaciformis L. plant is a kind of halophyte that is ableto survive in high salt conditions. It is important to determine itsphysiological, biochemical and molecular limit of NaCl stress if one aims touse it for phytoremediation purpose. In this study, the alkaline protocol ofthe modified plant comet assay were used for rapid detection of DNA damage inC. acinaciformis L. plants exposed to a series of NaCl stress concentrations (0-, 50-, 100-,200-, 300-, 400 and 500 mmol L-1) in hydroponic conditions for 2weeks. DNA damage was measured as the values of percentage of DNA in tails andtail length. The halophyte C. acinaciformis L. did not show any dose responseup to 400 mmol L-1 NaCl level in terms of DNA damages. DNA integritymeasured via comet assay showed that DNA preserved its original shape up to 400mmol L-1 NaCl level. However, the very high concentrations of NaCl(400 and 500 mmol L-1) caused DNA damages. When physiological and biochemical parameterssuch as proline, chlorophyll a and b, peroxidase (POX), catalase (CAT), H2O2,malondialdehyde (MDA) contents were examined, oxidant molecules such as H2O2(0.912-3.72 µmol g-1 Fwt) and MDA (7.1-34 nmol g-1 Fwt)gradually increased along with the increase of NaCl concentrations, p<0.05.On the other hand, antioxidant enzyme POX and an osmolyte molecule prolineslightly increased up to 400 mmol L-1 NaCl level then slightlydecreased after that. Similar issues were obtained from that of protease enzymewhich indicates the power of protein hydrolysis in which a slight decrease(182-95 Unit mg-1 protein) over a dose of NaCl was evident.Chlorophyll contents and CAT activity were not affected upon increase of NaClconcentrations. This study showed that the halophyte C. acinaciformis L. can beeasily used to remove salt up to 400 mmol L-1 NaCl concentrationsfrom a saline-affected soil. Measuring DNA damage is concluded as a very usefulparameter to find out what level of NaCl could be tolerated if a halophyteplant is aimed to remediate the saline soils.
KW  - Halophyte
KW  - NaCl stress
KW  - SCGE
KW  - Comet assay
KW  - DNA damage
N2  - Carpobrotus acinaciformis L. bitkisi yüksek tuz koşullarında yaşayabilen birçeşit halofit bitkidir. Bu bitki fitoremediasyonçalışmaları için kullanılmak üzere planlandığında, bu bitkinin tuz stresinekarşı fizyolojik, biyokimyasal ve moleküler sınırlarını belirlemek önem arzetmektedir. C.acinaciformis L.bitkisinde DNA hasar seviyesini belirlemek için hidroponik koşullarda 2 haftasüre ile tuz stresine (0-, 50-, 100-,200-, 300-, 400 and 500 mmol L-1)maruz bırakılan bitkilerde modifiye edilmiş alkali bitki comet assay metodukullanılmıştır. DNA hasarı kuyruk uzunluğu ve kuyrukta DNA yüzdesi olarak ölçülmüştür.Halofit C.acinaciformis L 400 mmol L-1 NaClseviyesine kadar DNA hasarı ile ilgili olarak doz tepkisi göstermemiştir. Cometassay ile ölçülen yönteme göre halofit bitkilerin DNA bütünlüğünü 400 mmolL-1 NaCl seviyesine kadar korunduğu gözlenmiştir. Fakat,çok daha yüksek NaCl konsantrasyonları (400 ve 500 mmol L-1)DNA hasarına yol açmıştır. Prolin, klorofil ave b, peroksidaz (POX), katalaz(CAT), H2O2, malondialdehid (MDA) içerikleri gibifizyolojik ve biyokimyasal parametreler incelendiğinde, oksidant moleküllerdenH2O2 (0.912-3.72 µmol g-1 taze ağırlık)andMDA (7.1-34 nmol g-1 taze ağırlık) artan tuz konsantrasyonu ileparalel olarak sıralı artış göstermiştir, p<0.05. Diğer yandan, antioksidant enzimlerden POX ve bir osmolit olan prolin 400mmol L-1 NaCl’ e kadarhafifçe artış göstermiş daha sonra tekrar düşmüştür. Benzer durumlar proteinhidrolizini belirlemede kullanılan proteaz enzim (182-95 Unit mg-1protein) seviyesinde de görülmüş, artanNaCl dozuna bağlı olarak enzim miktarı kademeli olarak azalmıştır. Klorofilmiktarı ve CAT enzim seviyesi NaCI konsantrasyon artışına bağlı olarak değişimgöstermemiştir. Bu çalışma, C. acinaciformis L. bitkisinin tuzdanetkilenmiş topraklarda 400 mmol L-1 NaCl’ e kadar olan tuz konsantrasyonunuuzaklaştırmada rahatlıkla kullanılabileceğini ortaya koymuştur. DNA hasarınıölçmek, tuzlu alanları ıslah etmede kullanılacak halofit bitkinin hangiseviyede NaCl stresine dayanabileceğini belirlemede çok kullanışlı birparametre olarak kaydedilmiştir.
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UR  - https://doi.org/10.29050/harranziraat.464133
L1  - http://dergipark.org.tr/tr/download/article-file/676023
ER  -