@article{article_513011, title={The Investigation of the Susceptibility of Candida Species to Fluconazole, Voriconazole, and Amphotericin B by Disc Diffusion and the Microdilution Method}, journal={Osmangazi Tıp Dergisi}, volume={42}, pages={444–449}, year={2020}, DOI={10.20515/otd.513011}, author={Sav, Hafize and Barış, Ayşe and Turan, Deniz and Özakkaş, Fatma and Altınbaş, Rabiye and Şen, Sümeyye and Kiraz, Nuri Kiraz}, keywords={Antifungal duyarlılık, Antifungal, Sıvı Mikrodilüsyon}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;line-height:normal;">In
this study, we aimed to investigate the antifungal susceptibility profile of
Candida isolates obtained in our hospital with two methods. A total of 200
Candida isolates were obtained from samples of patients in various departments
of our hospital. Identification of each strain was performed using conventional
(germ tube formation, microscopic morphology in corn meal-Tween 80 agar), and
commercial kit API 20C (Biomerieux, France). MIC values of fluconazole (FLU),
voriconazole (VORI), and amphotericin B (AMB) were evaluated according to
Clinical Laboratory Standards Institute broth microdilution and disk diffusion
(DD) method. Totally isolated 200 Candida species were identified as 100 C.
albicans, 25 C. glabrata, 7 C. krusei, 43 C. parapsilosis, and 25 C.
tropicalis. All isolates were susceptible to VORI. High resistance rate (22,5%)
was detected for AMB antifungal by reference microdilution method. Between two
methods, the best categorical ratio was calculated as VORI (99%), FLU (84.9%),
and AMB (72,5%) respectively. Voriconazole exhibited better activity in vitro
than fluconazole, even in isolates fluconazole resistant and disk diffusion
method can be considered as alternative antifungal sensitivity method for VORI. </p> <p> </p>}, number={4}, publisher={Eskişehir Osmangazi Üniversitesi}