TY - JOUR T1 - Comparison of the Aflatoxin levels in Fish Feeds from Rainbow Trout Farms Localized at Different Regions (Adana-Ağrı, TURKEY) TT - Farklı Bölgelerde (Adana-Ağrı, TÜRKİYE) Bulunan Gökkuşağı Alabalığı Çiftliklerindeki Balık Yemlerinde Aflatoksin Düzeylerinin Karşılaştırılması AU - Cengizler, İbrahim AU - Yakan, Selvinaz AU - Duman, Selçuk AU - Etyemez Büyükdeveci, Miray AU - Aldık, Ruhay PY - 2019 DA - December Y2 - 2019 DO - 10.29132/ijpas.522652 JF - International Journal of Pure and Applied Sciences PB - Munzur Üniversitesi WT - DergiPark SN - 2149-0910 SP - 78 EP - 84 VL - 5 IS - 2 LA - en AB - Aflatoxins levels in different fish feedsobtained from two different rainbow trout (Oncorhynchusmykiss) farms located in Adana and Ağrı regions were determined between Mayand July 2017. During the experiment period, ''a drought status'' was reportedaccording to the Standardized Precipitation Index of Turkey by Turkish StateMeteorological Service. According to precipitation index records, while Adanawas reported as slightly humid, Ağrı was slightly dry in the period between Mayand July 2017. The aflatoxin analyses of fish feed samples was carried out inthe laboratories of the Ministry of Food, Agriculture and Livestock in Adana,Turkey. The aflatoxin levels of the samples were resolved by liquid-solidextraction, immune-affinity pillar clean-up using a HPLC-FD (High PerformanceLiquid Chromatography with Fluorescence Detection). The detection limits ofaflatoxin were 0.50, 0.23, 0.73, 0.20 ppb for AFB1, AFB2, AFG1, AFG2,respectively. The results showed that, aflatoxin levels of fish feeds werebelow the detection limits in all samples.Aflatoxins levels in different fish feedsobtained from two different rainbow trout (Oncorhynchusmykiss) farms located in Adana and Ağrı regions were determined between Mayand July 2017. During the experiment period, ''a drought status'' was reportedaccording to the Standardized Precipitation Index of Turkey by Turkish StateMeteorological Service. According to precipitation index records, while Adanawas reported as slightly humid, Ağrı was slightly dry in the period between Mayand July 2017. The aflatoxin analyses of fish feed samples was carried out inthe laboratories of the Ministry of Food, Agriculture and Livestock in Adana,Turkey. The aflatoxin levels of the samples were resolved by liquid-solidextraction, immune-affinity pillar clean-up using a HPLC-FD (High PerformanceLiquid Chromatography with Fluorescence Detection). The detection limits ofaflatoxin were 0.50, 0.23, 0.73, 0.20 ppb for AFB1, AFB2, AFG1, AFG2,respectively. The results showed that, aflatoxin levels of fish feeds werebelow the detection limits in all samples. KW - Aflatoxin KW - Fish feed KW - HPLC analysis KW - Rainbow trout farm N2 - Mayıs ve Temmuz 2017 tarihleri arasındaAdana ve Ağrı bölgelerinde bulunan iki farklı gökkuşağı alabalığı (Oncorhynchus mykiss) çiftliğinden eldeedilen farklı balık yemlerinde aflatoksin seviyeleri belirlendi. Çalışmamızaait dönemde Türkiye aylık standart yağış indeksine göre kuraklık durumukaydedilmiştir. Kayıtlarımıza göre Ağrı ili Mayıs-Temmuz aylarında hafif kurak,Adana ili ise hafif nemli bir hava göstermiştir. Yem örneklerinin analizi T.C.Adana Gıda Tarım ve Hayvancılık Laboratuvarlarında yürütülmüştür. Numunelerfloresan saptama (HPLC-FD) ile yüksek performanslı sıvı kromatografisikullanılarak, sıvı-katı özütleme immune-affinity column clean-up metodu ilebelirlenmiştir. Aflotoksin tespit limiti AFB1, AFB2, AFG1, AFG2 için sırasıyla0,50; 0,23; 0,73; 0,20 ppb, olarak belirlenmiştir. Örneklenen tüm balıkyemlerinde aflatoksin düzeylerin tespit edilebilir değerin altında olduğubulunmuştur. 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