TY - JOUR T1 - FARKLI KÖK KANAL PATLARININ SİTOTOKSİSİTE YÖNÜNDEN KARŞILAŞTIRILMASI AU - Şahin, Meşkule AU - Aslan, Berna AU - Yılmaz, Funda PY - 2019 DA - January DO - 10.17567/ataunidfd.522728 JF - Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi JO - Ata Diş Hek Fak Derg PB - Atatürk Üniversitesi WT - DergiPark SN - 1300-9044 SP - 66 EP - 73 VL - 29 IS - 1 LA - tr AB - Amaç:Kök kanal materyalleri periapical dokular üzerinde çeşitli derecelerdesitotoksisite göstermektedir. Bu durum sonucunda sırasıyla; enflamasyonoluşumu, yara iyileşmesinin gecikmesi ve kemik rezorbsiyonu oluşmaktadır. Kökkanal dolgu materyalleri doğrudan canlı dokularla temas halinde oldukları içinbiyolojik olarak periapikal dokularla uyumlu olmalıdır. Çalışmamızın amacı,farklı içeriklere sahip kök kanal dolgu patlarının L-929 hücre süspansiyonudakisitotoksik etkilerinin karşılaştırılmasıdır. Gereç veYöntem: AH Plus, Tubli Seal EWT, EndoREZ, MTA Fillapex,Real Seal’in üreticilerin talimatları doğrultusunda steril şartlarda karıştırılıphazırlandı. Hazırlanan örnekler 6 kuyucuklu plağın her bir kuyucuğunda farklıkök kanal dolgu patı olacak şekilde yerleştirildi. Örnekler üzerine DMEM ilaveedilerek, 24, 48 ve 72 saat bekletildi. Elde edilen ekstraksiyon sıvıları,L-929 hücre süspansiyonu içeren 96 kuyucuklu plakların her bir gözüne 100 µlyerleştirildi. 48 saatlik inkübasyondan sonra plaklardan ekstraksiyon sıvılarıuzaklaştırılmış ve her bir göze 25 µl MTT solüsyonu ilave edilerek 4 saat süreile bekletilmiştir. Bu sürenin sonunda spektrofotometrede 570 nm’deabsorbansları ölçülmüştür.Bulgular:24, 48 ve 72 saatlik değerlendirmede AH Plus ve kontrol grubu arasındaistatistiksel olarak anlamlı bir fark gözlenmezken (p>0,05), diğer kök kanaldolgu patları ve kontrol grubu arasında istatistiksel olarak anlamlı bir farkvardır (p<0,05). Kontrol grubuna ait hücre canlılık oranları Tubli Seal EWT,EndoREZ, MTA Fillapex ve  Real Seal kökkanal dolgu patlarının hücre canlılık oranlarından anlamlı derecede daha yüksektir.Sonuç:AH Plus 3 zaman diliminde de sitotoksisite göstermezken, Tubli Seal EWT,EndoREZ, MTA Fillapex ve Real  Seal 24. ve 48. saatlerde orta, 72.saatte ise hafif sitotoksisite göstermişlerdir. Anahtar Kelimeler: Kök kanal dolgu patları, MTT testi, sitotoksisitetCOMPARISION OF CYTOTOXICITY OF DIFFERENT ROOT CANALSEALERSABSTRACT Aim: Root canal sealersexhibit varying degrees of cytotoxicity to periapical tissues. This in turnresults in inflammation, delayed wound healing, and even bone resorption. Root canal sealers were contact with periapical tissuesand must be biocompatable. The aim of this study was to compare the cytotoxiceffect of different root canal sealers in different contents on L-929 cellsuspension. Materyaland Methods: AH Plus, Tubli Seal EWT, EndoREZ, MTA Fillapex andReal Seal were mixed under sterilized condition according to manufacturer’sinstructions. Mixed sealers were placed in six-well plate and every sealersplaced at the bottom of each well to cover an area of 1 cm diameter. PlacedDMEM on samples for 24, 48 and 72 hours. 100 µl of eluate was transferred to a96- well plate containing L-929 cell suspension. After 48 hours incubation theextracts were removed from the wells, 25 µl MTT solution was added to each welland waited for 4 hours. Subsequently, the spectrophotometric absorbance wasmeasured at 570 nm using a spectrophotometer.Results:According to the MTT assay, in the rates of cell viability there was nostatistically significant difference between AH Plus and control group at 24,48 and 72-hour evaluation (p>0.05), but there was statistically significantdifference between the other sealers and control group (p<0.05). The controlgroup had significantly high rates of cell viability than Tubli Seal EWT,EndoREZ, MTA Fillapex and Real Seal.Conclusion:For each 24, 48 and 72 hours AH Plus was non-cytotoxic, Tubli Seal EWT,EndoREZ, MTA Fillapex and Real Seal had moderate cytotoxicity at 24 and 48 hourevaluation and slightly cytotoxicity at 72 hour evaluation.Key Words:Cytotoxicity, MTT assay, root canal sealers  KW - Kök kanal dolgu patları KW - MTT testi CR - 1. Himel VT, Mcspadden JT, Goodis HE. Instruments, materials, and devices. In: Pathways of the Pulp. “9nd Ed.” Cohen, S., Hargreaves, K.M., St. Louis: Mosby 2006: p.233-290. CR - 2. Schmalz G, Arenholt-Bindslev D. Root canal filling materials. In: Textbook of Endodontology “2th ed.” Ed. Bergenholtz, G., Hørsted-Bindslev, P., Reit, C., West Sussex. John Wiley & Sons. 2010: p.193-218. CR - 3. Kaur A., Shah N., Logani A., Mishra N. Biotoxicity of commonly used root canal sealers: a meta-analysis. J Conserv Dent 2015;18:83-8. CR - 4. 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