
   @article{article_529237, title={Transposon studies on Colchium chalcedonicum}, journal={International Journal of Life Sciences and Biotechnology}, volume={2}, pages={25–35}, year={2019}, DOI={10.38001/ijlsb.529237}, author={Karlık, Elif and Albayrak, Merve and Uzen, Erdal and Gözükırmızı, Nermin}, keywords={Colchicum chalcedonicum,Retrotransposons,SIRE1,Sukkula,Nikita}, abstract={&amp;lt;div style="border:solid 1pt;padding:1pt 4pt 1pt 4pt;"&amp;gt; &amp;lt;p class="Default" style="text-align:justify;border:none;padding:0cm;"&amp;gt; &amp;lt;i&amp;gt; &amp;lt;span lang="en-gb" style="font-size:10pt;font-family:’Times New Roman’, serif;" xml:lang="en-gb"&amp;gt;Colchicum chalcedonicum &amp;lt;/span&amp;gt; &amp;lt;/i&amp;gt; &amp;lt;span lang="en-gb" style="font-size:10pt;font-family:’Times New Roman’, serif;" xml:lang="en-gb"&amp;gt;is one of
the endemic plants in Turkey. The aim of this study was the investigation of
the retrotransposon &amp;lt;i&amp;gt; SIRE1, Sukkula &amp;lt;/i&amp;gt;
and &amp;lt;i&amp;gt;Nikita &amp;lt;/i&amp;gt; presence and insertion
patterns in &amp;lt;i&amp;gt;C. chalcedonicum. &amp;lt;/i&amp;gt;The
plant samples were collected from the botanic garden of the Istanbul
University. DNA isolation was performed from leaves by using modified
CTAB/SEVAG protocol. Retrotransposon movements were investigated using &amp;lt;i&amp;gt;SIRE1, Sukkula &amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Nikita &amp;lt;/i&amp;gt;primers by Inter Retrotranposon Amplified Polymorphism PCR
technique (IRAP-PCR). Polymorphism percentages (%) were calculated based on
Jaccard Similarity Index. We observed that polymorphism ratios of &amp;lt;i&amp;gt;SIRE1 &amp;lt;/i&amp;gt;, &amp;lt;i&amp;gt;Sukkula &amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Nikita &amp;lt;/i&amp;gt;
retrotransposons among all samples were 0-40%, 0-100% and 0-60%, respectively.
This is the first report to demonstrate three barley ─ &amp;lt;i&amp;gt; SIRE1 &amp;lt;/i&amp;gt;, &amp;lt;i&amp;gt;Sukkula &amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Nikita─ &amp;lt;/i&amp;gt; retrotransposons presence and
movements in &amp;lt;i&amp;gt;C. chalcedonicum &amp;lt;/i&amp;gt;which
is belonged to &amp;lt;i&amp;gt; Colchium &amp;lt;/i&amp;gt;family, thus &amp;lt;i&amp;gt; &amp;lt;/i&amp;gt;these IRAP primers may be
used in further characterization and diversity studies of &amp;lt;i&amp;gt;Colchicum &amp;lt;/i&amp;gt;
family.  &amp;lt;b&amp;gt; &amp;lt;/b&amp;gt; &amp;lt;/span&amp;gt; &amp;lt;/p&amp;gt; &amp;lt;p&amp;gt; &amp;lt;b&amp;gt; &amp;lt;/b&amp;gt; &amp;lt;/p&amp;gt; &amp;lt;b&amp;gt; &amp;lt;/b&amp;gt; &amp;lt;p class="Default" style="text-align:justify;border:none;padding:0cm;"&amp;gt; &amp;lt;b&amp;gt; &amp;lt;span lang="en-gb" style="font-size:10pt;font-family:’Times New Roman’, serif;" xml:lang="en-gb"&amp;gt; &amp;lt;/span&amp;gt; &amp;lt;/b&amp;gt; &amp;lt;/p&amp;gt; &amp;lt;p&amp;gt; &amp;lt;b&amp;gt;  &amp;lt;/b&amp;gt; &amp;lt;/p&amp;gt; &amp;lt;b&amp;gt; &amp;lt;/b&amp;gt; &amp;lt;/div&amp;gt;}, number={1}, publisher={International Society of Academicians}