TY - JOUR T1 - Bacillus subtilis Bakterisi ile Metribuzin Herbisitinin Biyoıslahının Yapay Tarla Düzeneğinde Araştırılması TT - Investigation of the Bioremediation of Herbicide Metribuzin with Bacillus subtilis Bacteria at Artifical Agricultural Field AU - Ergüven, Gökhan Önder PY - 2019 DA - July Y2 - 2019 DO - 10.29132/ijpas.529882 JF - International Journal of Pure and Applied Sciences PB - Munzur Üniversitesi WT - DergiPark SN - 2149-0910 SP - 46 EP - 52 VL - 5 IS - 1 LA - tr AB - Türkiye’de domates yetiştiriciliğinde seçkin birherbisit olan Metribuzinin (C8H14N4OS) biyoıslahıBacillus subtilis bakterisiyleçalkalanmalı kültür koşullarında ve yapay tarla düzeneğine araştırılmıştır.Bakteri Türkiye Elazığ ilinden bir tarım arazisinden toplanan topraktan izoleedilmiştir. Bölge daha önce metribuzine maruz kalmayan bir bölgedir. 1750 mg L-1konsantrasyonda (çiftçler için tavsiye edilen konsantrasyon) 100’er mL’lik beş adet aparat hazırlanmıştırve metribuzin her bir erlenmayer şişesine ilave edilmiştir. Her birzenginleştirilen solüsyon ×107 Koloni oluşturan birey (KOB) içermektedir. Bu şişeler 160 rpm de28°C’de steril koşullarda 17 gün boyuncaçalkalanmıştır. 24 saatlik dilimlerde, her bir örnek toplanmıştır ve DR/890kolorimetre ile kimyasal oksijen ihtiyacı (KOİ) kapalı refluks yöntemi ışığındabelirlenirken Biyokimyasal Oksijen İhtiyacı (BOİ5) standart method5210D ye göre oksitoplarla belirlenmiştir. Çalışma sonuçlarına göre en iyigiderim performansı 100 mL Bacillussubtilis türünde KOİ ve BOİ5 bazında 17 günde %98 oranındagörülmüştür. Yapay tarla çalışmasının sonuçlarına göre KOİ ve BOİ5 parametrelerinde2. haftanın sonunda sırasıyla %99.4 ve %96 olarak görülmüştür. Bu deneylergöstermiştir ki Bacillus subtilis alıcıortamlardaki metribuzin gieriminde seçkin bir türdür. KW - Bakteri KW - biyoıslah KW - herbisit KW - metribuzin N2 - The bioremoval ofmetribuzin (C8H14N4OS) which is one of theselective herbicide in tomato farming in Turkey was investigated with Bacillus subtilis bacteria underagitated culture conditions and artifical agricultural field. The bacterium wasisolated in collected soil samples from one of the agricultural area in Elazigprovince of Turkey. The area is unexposed to metribuzin before. Fiveapparatuses were set up and 100 mL of metribuzin in 1750 ppm concentration(advised concentration of tomato farmers) was added to each Erlenmayer flasks.5, 10, 20, 50 and 100 mL of enriched Bacillussubtilis solutions (in sabouraud dextrose broth) added to these flasks.Each mL of the enriched solutions contains ×107 Colony forming unit (CFU).These flasks were shaken at 160 rpm at 28 0C in sterile conditionsfor 17 days. 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