TY - JOUR T1 - MCF7 meme kanseri hücreleri üzerine Verbascum inulifolium’un antiproliferatif ve apoptoz indükleme aktivitelerinin değerlendirilmesi TT - Antiproliferative and apoptosis inducing effect of Verbascum inulifolium on MCF-7 breast cancer cell line AU - Özay, Yusuf AU - Yıldız, Atila AU - Güven, Celal AU - Albeniz, İşıl AU - Türker Şener, Leyla AU - Aydoğan Ahbab, Mufide AU - Bozgeyik, İbrahim AU - Güzel, Sevda AU - Bağış, Haydar AU - Yumrutaş, Önder PY - 2019 DA - May DO - 10.31020/mutftd.532710 JF - Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi PB - Mersin Üniversitesi WT - DergiPark SN - 1309-8004 SP - 203 EP - 210 VL - 9 IS - 2 LA - tr AB - Verbascum cinsi halk tıbbında geleneksel olarak kullanılanve anti-enflamatuvar, antioksidan ve antibakteriyal aktiviteleri bilinen bircinstir. Verbascum inulifolium türüTürkiye’de yetişen endemik bir türdür. Bu çalışmanın amacı V. inulifolium türünün toprak üstü kısımlarından elde edilenmetanol ekstresinin insan meme kanseri hücre hattı (MCF7) üzerineantiproliferatif ve apoptotik etkilerinin değerlendirilmesidir. Ekstreninfarklı konsantrasyonlarının (1, 5, 25 ve 125µg/ml) hücre canlılığı veproliferasyonu üzerine etkisi MTT ve Xcellange yöntemi ile test edilmiştir.Daha sonra hücreler Annexine V/propodium iodür (PI) ile boyanmış ve apoptozunsafhaları FACS cell counter metodu ile belirlenmiştir. Ayrıca apoptotik veanti-apoptotik protein ekspresyon düzeyleri western blot metodu ilebelirlenmiştir. Son olarak phallodine boyama metodu ile hücrelerde aktinflamentleri boyanmış ve floresan mikroskobunda incelenmiştir. MCF-7 hücrecanlılığı ve proliferasyonun doz artışına bağlı olarak azaldığı belirlenmiştir.Ancak apoptozun erken veya geç dönemlerinde MCF-7 hücre sayılarında bir değişimgözlenmemiştir. P53, Bcl-2 ve kaspaz-3 protein ekspresyon düzeylerinde anlamlıbir değişim gözlenmemiştir. Ayrıca aktin flamentlerinin yoğunluğu sadece 125mg/ml dozda hafif azalmıştır. Sonuç olarak V.inulifolium türünün methanol ekstresi MCF-7 hücreleri üzerineanti-proliferatif etki göstermiştir, fakat sonuçlar bu etkinin apoptotikyolakla ilişkili olmadığı göstermiştir. KW - Verbascum inulifolium KW - breast cancer KW - antiproliferation KW - KW - apoptozis N2 - The genus Verbascumtraditionally used in folk medicine is known with some activities includinganti-inflammatory, antioxidant and antibacterial. Verbascum inulifolium is an endemic species growing in Turkey. Theaim of the present study was to evaluate anti-proliferative and apoptoticeffects of methanol extract obtained from aerial parts of V. inulifolium on human breast cancer cellline (MCF). Effects of different concentration of the extract (1, 5, 25 and125µg/ml ) were tested on cell viability and proliferation using MTT andXcelligence assays. Then cells stained with Annexine V/propodium iodür (PI) andapoptosis phases were determined using FACS cell counter method. Moreover,western-blot method was performed to determine apoptotic and anti-apoptoticprotein expression levels. Finally, cell actin filaments stained usingphallodine stain method and examined with fluorescence microscope. Cellviability and proliferation levels were reduced according to increasingconcentrations of the extract. However, there were no differences observed innumber of MC-7 cell during early and late phases of apoptosis. There were nosignificantly differences observed in P53, Bcl-2, and caspase-3 proteinexpression levels. Actin filament density was slightly reduced only at the concentrationof 125 mg/ml. As a result, methanol extract of V. inulifolium showed anti-proliferative effect, but results indicatedthat this effect was not related to apoptotic pathway. CR - Elmore S. 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