TY  - JOUR
T1  - Maya mantarlarının identifikasyonunda iki farklı kromojenik besiyerinin karşılaştırılması
TT  - COMPARISON OF TWO CHROMOGENIC MEDIA FOR YEAST IDENTIFICATION
AU  - Ergon, M. Cem
AU  - Tunç, Buğse
AU  - Doluca Dereli, Mine
PY  - 2018
DA  - May
DO  - 10.5505/deutfd.2018.26121
JF  - Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi
JO  - J DEU Med
PB  - Dokuz Eylül Üniversitesi
WT  - DergiPark
SN  - 2602-3148
SP  - 23
EP  - 31
VL  - 32
IS  - 1
LA  - tr
AB  - Amaç: Kateter, protez kullanımı,uzun süren geniş spektrumlu antibiyotik tedavileri, organ nakli ve immünyetersizlik gibi faktörler invazif Candida enfeksiyonlarında artışa nedenolmaktadır. Son yıllarda hızlı maya identifikasyonunu sağlamak amacıyla birçok kromojenikbesiyeri geliştirilmiştir. Bu çalışmada maya suşlarının tür düzeyindetanımlanmasında iki farklı kromojenik besiyeri olan CHROMagar Candida(CHROMagar, Fransa) ile Rosachrom Candida Agar II’nin (Gül BiyolojiLaboratuvarı, Türkiye) performanslarının karşılaştırılması amaçlandı.Gereç ve Yöntem: Çalışmaya, Eylül – Ekim 2015tarihleri arasında hastanemiz Mikoloji Laboratuvarında çeşitli klinikörneklerden soyutlanan 22 C. glabrata,16 C. tropicalis, 10 C. albicans, dokuz C. parapsilosis, dört C. kefyr, iki Saprochaete capitata, iki Trichosporonspp., bir C. lusitaniae olmaküzere toplam 66 suş alındı. Suşların her iki kromojenik besiyerindeki kolonimorfolojisi ile rengi ve koloni etrafında halenin varlığı, 24. ve 48. saatlerdeiki farklı kişi tarafından değerlendirildi.Bulgular: Çalışmaya alınan izolatlarıntümü her iki kromojenik besiyerinde de ayırt edilebilen koloniler şeklinde iyiüreme gösterdi. C. albicans için24.-48. saatlerde duyarlılık ve özgüllük değerleri sırası ile CHROMagar Candidaiçin %100 ve Rosachrom Candida için %70-80 ile %94,6-96,4 olarak saptandı. Budeğerler, C. tropicalis izolatlarındasırası ile CHROMagar Candida için %100; Rosachrom Candida için %43,7–50 ve%98-100 idi.Sonuç: CHROMagar Candida, C. albicans, C. tropicalis ve C. glabratatürlerini doğru identifiye etmede gösterdiği yüksek duyarlılık ve özgüllükoranları nedeniyle Candida türlerinin identifikasyonu için uygun bir besiyeriolarak belirlendi. Rosachrom Candida besiyerinin ise, C. albicans identifikasyonunda CHROMagar Candida besiyerinealternatif olarak düşünülebileceği ancak C.tropicalis identifikasyonundaki performansının yeterli olmadığı görüşünevarıldı.
KW  - Maya Mantarı İdentifikasyonu
KW  - Kromojenik Besiyeri
KW  - Chromagar Candida
KW  - Rosachrom Candida
N2  - Objective: Usage of catheter, prosthesis, treatment with extendedspectrum antibiotics for a long time, organ transplantation andimmunodeficiency enhanced invasive Candida infections. Quite a few chromogenic mediawere developed for rapid identification of yeasts, recently. We aimed tocompare performances of CHROMagar Candida (CHROMagar, France) and RosachromCandida Agar II (Gül Biyoloji Laboratuvarı, Turkey) for the identification ofthe yeast species.Material and Method: Totally 66 isolates including 22 C. glabrata, 16 C. tropicalis,10 C. albicans, nine C. parapsilosis, four C. kefyr, two Saprochaete capitata, two Trichosporonspp., one C. lusitaniae isolatedfrom various clinical specimens between September–October 2015 in MycologyLaboratory of our hospital were included. The colonies of the isolates wereevaluated by considering the colony morphology, color and the existence of haloaround the colony by two different people after 24 and 48 hours.Results: The isolates grew well on both chromogenic media, andshowed clearly distinguished colonies. The sensitivity and specificity valuesfor C. albicans were detected as 100%for CHROMagar Candida; 70-80% and 94.6-96.4% for Rosachrom Candida, at 24 and48 hours, respectively. These values for C.tropicalis were 100% for CHROMagar Candida; 43.7–50 % and 98-100 % forRosachrom Candida, respectively.Conclusion: CHROMagar Candida whichcan identify, C. albicans, C. tropicalis and C. glabrata with high sensitivity and specificity rates wasconcluded as a reliable medium for identification of Candida species. RosachromCandida may be thought to be an alternative to CHROMagar Candida for C. albicans identification but itsperformance in identification of C.tropicalis was not found sufficient.
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UR  - https://doi.org/10.5505/deutfd.2018.26121
L1  - http://dergipark.org.tr/tr/download/article-file/665379
ER  -