TY  - JOUR
T1  - Kültüre Edilen Sivriburun Karagöz Balıklarında (Diplodus puntazzo Cetti, 1777) Micrococcus luteus  Enfeksiyonunun Teşhisi
TT  - Diagnosis of Micrococcus luteus Infection in Cultured Sharpsnout Sea Bream (Diplodus puntazzo Cetti, 1777)
AU  - Yardımcı, Remziye Eda
AU  - Akaylı, Tülay
AU  - Ürkü, Çiğdem
PY  - 2020
DA  - August
JF  - Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi
PB  - Erciyes Üniversitesi
WT  - DergiPark
SN  - 1012-2354
SP  - 214
EP  - 220
VL  - 36
IS  - 2
LA  - tr
AB  - Buçalışmada Ege Denizi'nde kültüre edilen sivriburun karagöz balıklarında (Diplodus puntazzo Cetti, 1777) meydanagelen ölümlerin nedeninin belirlenmesi amaçlandı. Mayıs 2009 ve Mayıs 2010tarihleri ​​arasında bakteriyolojik yöntemler kullanılarak toplam 10 hastabirey (200-350g) incelendi. Hasta balık örneklerinde solgun solungaç vekaraciğer, deri renginde açılma, karaciğerde ve kaslarda kanamalar görüldü.Hasta balıkların karaciğer, böbrek ve dalağından Marine Agar (MA) ve % 1,5 NaCliçeren Tryptic Soy Agar (TSA) yapılan bakteriyolojik ekimler 22 ° C'de 48 saatsüreyle inkübe edildi. Gram pozitif hareketli olmayan koklar viseralorganlardan izole edildi. Morfolojik ve biyokimyasal özelliklerine göre, buizolatlar Miccrococcus luteus olarak identifiye edildi. Kirby-Bauerdisk difüzyon yöntemi çoklu disk kullanılarak yapıldı. Sonuçlar mevcutverilerine dayanılarak yorumlandı ve tüm izolatların oksitetrasiklin,eritromisin ve kloramfenikole karşı duyarlı olduğu belirlendi. Histopatolojikolarak karaciğer parankim hücrelerinde vakuolar dejenerasyon ve nekroz,böbreklerde tübüler nekroz ve dalakta hemosiderin depozitleri saptandı. Lamellalardaerimenin yanı sıra solungaçlarda epitel hücre hiperplazisi gözlendi. Sonuçolarak, bu çalışmada M. luteus’un neden olduğu bir düşük mortaliteli bir epizootik tanımlandıve bu çalışma bu patojenin kültür sivriburun karagöz balıklarında tanımlandığı ilkrapordur.
KW  - Sivriburun Karagöz
KW  - Kafes Yetiştiriciliği
KW  - Micrococcus luteus
N2  - The aim of the study was to determinate the cause ofmortalities in cultured sharpsnout sea bream (Diplodus puntazzo Cetti, 1777) in the Aegean Sea. Ten individuals(200-350 g) in total between May 2009 – 2010 were investigated usingbacteriological methods. Moribund fish samples exhibited pale gills and liver,skin depigmentation, haemorrhaging in the liver and muscles. Bacteriologicalsamples of liver, kidney and spleen were streaked onto Marine Agar (MA) andTryptic Soy Agar (TSA) plates containing %1,5 NaCl and incubated at 22°C for48h. Gram-positive non-motile cocci were isolated from visceral organs.According to their morphological and biochemical characteristics, theseisolates were identified as Micrococcusluteus. The Kirby-Bauer diskdiffusion method was also performed using multidisc. Results were interpretedbased on the available data and all isolates were determined to be sensitiveagainst oxytetracycline, erythromycin and chloramphenicol. Histopathologically,vacuolar degeneration and necrosis in the parenchyma cells of liver, tubularnecrosis in the kidney and hemosiderin deposits in the spleen were determined.In addition to melting lamella, epithelial cells hyperplasiain the gills was observed. In conclusion, in this study an epizootic with lowmortality caused M. luteus weredescribed and this study is the first report of the pathogen in the culturedsharpsnout sea bream.
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UR  - https://dergipark.org.tr/tr/pub/erciyesfen/issue//537290
L1  - https://dergipark.org.tr/tr/download/article-file/1245467
ER  -