TY  - JOUR
T1  - Kışlık Buğdaylarda Kök ve Kökboğazı Çürüklüğüne Sebep Olan Fusarium spp. İzolatları Arasındaki Genetik Varyasyonun Retrotranzpozon Temelli iPBS Markörleri ile İncelenmesi
TT  - Genetic Variation of Fusarium spp. Isolates Associated with Root and Crown Rot of Winter Wheat Using Retrotransposon-Based iPBS Assays
AU  - Özer, Göksel
AU  - Alkan, Mehtap
AU  - Göre, Mehmet Erhan
AU  - Bayraktar, Harun
PY  - 2019
DA  - December
Y2  - 2019
DO  - 10.24180/ijaws.537423
JF  - Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi
PB  - Bolu Abant İzzet Baysal Üniversitesi
WT  - DergiPark
SN  - 2149-8245
SP  - 250
EP  - 259
VL  - 5
IS  - 2
LA  - tr
AB  - Bu çalışmada,kışlık buğdaylarda kök ve kökboğazı çürüklüğüne sebep olan farklı Fusarium türlerine ait izolatlar arasındaki genetik çeşitlik,retrotranspozon temelli olan primerler arası bağlanma bölgesi (iPBS) markörlerikullanılarak incelenmiştir. Bu amaçla, 2017 ve 2018 yıllarında Türkiye veAzerbaycan buğday yetiştirme alanlarından 32 Fusarium türü izolat eldeedilmiştir. İzolatlar arasında en yaygınbulunan tür 23 izolat ile F. culmorumolarak belirlenmiş, bunu beş izolat ile F.pseudograminearum ve dört izolat ile F.graminearum takip etmiştir. Yedi iPBS retrotranspozon markörü izolatlar için99 adeti polimorfik (%86.8) olmak üzere 114 bant üretmiş, primer başınaortalama 14.14 polimorfik bant elde edilmiştir. Markörlerin polimorfik bilgiiçeriği (PIC) ortalama 0.17 olup, bu değer 0.10 ile 0.29 arasında değişmiştir. iPBSmarkörleri ile elde edilen verilere dayalı ağırlıklı olmayan aritmetik ortalamaeş grup metodu (UPGMA) küme analizlerinin sonrasında elde edilen dendrogramda,izolatlar tür seviyesinde ile üç gruba ayrılmıştır. Ayrıca grup içerisinde yeralan izolatlar ise orijini olan ülkeye göre dağılım sergilemiştir. Popülasyonyapısı, Bayesian modellemesine dayanılarak hesaplanmış ve sonuçlardendrogramdaki izolatların kümelenmesini destekleyecek şekilde üç popülasyonaayrılmış (K = 3) ve Ln olasılığınınen yüksek ortalama değeri (-476.0) eşlik etmiştir. iPBS markörlerininkullanılması, türler arası seviyede oldukça yüksek düzeyde polimorfizm üretmişolup türlerin birbirinden ayrılmasını sağlamıştır. Çalışma, buğdaydan eldeedilmiş Fusarium spp. izolatlarınıngenetik çeşitliliğini ve popülasyon yapısını iPBS markörleri analizi ile ortayakoyan ilk çalışma olma özelliği taşımaktadır.
KW  - Buğday
KW  - Fusarium spp.
KW  - iPBS markörleri
KW  - Retrotranspozon
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UR  - https://doi.org/10.24180/ijaws.537423
L1  - https://dergipark.org.tr/tr/download/article-file/881180
ER  -