@article{article_544067, title={An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients*}, journal={Anatolian Clinic the Journal of Medical Sciences}, volume={25}, pages={19–26}, year={2020}, author={Erimşah, Sevilay and Gonca, Süheyla and Kükner, Aysel and Duran, Bülent and Yazır, Yusufhan}, keywords={DNA bütünlüğü,intrauterin inseminasyon,mikroakışkan çip,sperm seçimi}, abstract={<p class="MsoNormal" style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en-us">Aim: </span> </b> <span lang="en-us" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en-us">In </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en-us">this study, we </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en-us"> evaluated the DNA integrity of sperms selected by using
the conventional </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us"> swim-up and
density gradient centrifugation techniques and the new nanotechnology-based microfluidic
chip method <span style="letter-spacing:-.1pt;">in order to determine the ideal
sperm selection method for the </span> </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en-us">intrauterine
insemination </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us"> </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">treatment of
normozoospermic infertile patients (NIPs). </span> </p> <p> </p> <pre style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;background:#FFFFFF;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Materials and Methods: </span> </b> <span lang="en-us" style="font-size:12pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">Semen samples obtained from 20 patients were divided into <span style="letter-spacing:-.1pt;">four equal fractions. Control, density gradient centrifugation, swim-up, and microfluidic </span> chip (MC) groups were created, and the untreated (control) and treated (other) sperm samples from the four groups </span> <span lang="en" style="font-size:12pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en">were examined for DNA integrity. A </span> <span lang="en-us" style="font-size:12pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us">cidic aniline blue <span style="letter-spacing:-.05pt;">staining and the TUNEL method were used respectively for evaluating sperm chromatin </span>condensation defects and DNA fragmentation. <span>Dichlorofluorescein diacetate </span> and flow cytometry were used to determine the reactive oxygen species levels. </span> <span lang="en-us" style="font-size:12pt;line-height:150%;font-family:’Times New Roman’, serif;" xml:lang="en-us"> </span> </pre> <p> </p> <p class="MsoNormal" style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;"> <b> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">Results: </span> </b> <span lang="en" style="font-family:’Times New Roman’, serif;" xml:lang="en">We measured significantly lower values of chromatin
condensation defects, DNA fragmentation and </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">reactive oxygen species </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us"> </span> <span lang="en" style="font-family:’Times New Roman’, serif;" xml:lang="en">for </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">the sperms
selected with the MC method, compared to the sperms </span> <span lang="en" style="font-family:’Times New Roman’, serif;" xml:lang="en">selected with the </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">conventional </span> <span lang="en" style="font-family:’Times New Roman’, serif;" xml:lang="en"> methods </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">(p<0.0001) </span> <span lang="en" style="font-family:’Times New Roman’, serif;" xml:lang="en">. </span> </p> <p> </p> <p class="MsoNormal" style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;"> </p> <p class="MsoNormal" style="margin-top:6pt;margin-right:0cm;margin-bottom:6pt;margin-left:0cm;text-align:justify;line-height:150%;"> <b> <span lang="en" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en">Discussion and Conclusion: </span> </b> <span lang="en" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en">We found that </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;letter-spacing:-.1pt;" xml:lang="en-us">the new MC method was more
effective in selecting </span> <span lang="en-us" style="font-family:’Times New Roman’, serif;" xml:lang="en-us">sperms with high
DNA integrity, compared to the conventional methods. Accordingly, the MC <span style="letter-spacing:-.1}, number={1}, publisher={Hayat Sağlık ve Sosyal Hizmetler Vakfı}