TY  - JOUR
TT  - Effects of 6-Shogaol, 6-Gingerol and Curcumin on Cell Viability in A549 Cancer Cell Line
AU  - Demirpolat, Eren
AU  - Yerer Aycan, Mükerrem Betül
PY  - 2017
DA  - March
JF  - Sağlık Bilimleri Dergisi
JO  - JHS
PB  - Erciyes Üniversitesi
WT  - DergiPark
SN  - 1018-3655
SP  - 24
EP  - 28
VL  - 26
IS  - 1
KW  - A549
KW  - 6-gingerol
KW  - 6-shogaol
KW  - kurkumin
KW  - IL-1β
N2  - In our study we investigated anticancer effects of 6- gingerol and 6-shogaol from Zingiber officinale. Anticancer effect was monitored by using cell viability assay. Experiments are performed on both A549 cells that are incubated with IL-1β (interleukin 1 β) and cells which are not incubated with it. Curcumin from Curcuma longa, a natural compound that is known for its anticancer effect, is used as a positive control and effects of 6- gingerol and 6-shogaol are compared with it. MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed for wide range concentrations. Concentration changes were 10 fold. To obtain IC50 values of 6-shogol and 6-gingerol, MTT assays were performed again, using the previous concentrations but in a narrower range.According to the assays 6-shogaol’s 24 h IC50 determined as 62,5 μM for A549 cells which were not incubated with IL-1β and 63,2 μM for the cells which were exposed to IL-1β. 6-shogaol’s cytotoxic effect was as much as curcumin which is a known natural anticancer molecule, while 6-gingerol did not show any effect towards decreasing the cell viability. Mechanisms which underly 6-shogoal’s cytotoxic effect are worth for further investigation.
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UR  - https://dergipark.org.tr/tr/pub/eujhs/issue//552941
L1  - https://dergipark.org.tr/tr/download/article-file/693670
ER  -