TY - JOUR T1 - The Association between Thiol-Disulphide Balance and CA-15-3 Levels in Patients with Breast Cancer TT - Meme Kanseri Hastalarında Dinamik Tiyol, Disülfit Dengesi ile CA-15-3 Seviyeleri Arasındaki İlişki AU - Özdemir, Ayşe AU - Dönem Dilli, Uttku AU - Özdemir, Dalyan AU - Neşelioğlu, Salim AU - Erel, Özcan PY - 2020 DA - March Y2 - 2019 DO - 10.18229/kocatepetip.560366 JF - Kocatepe Tıp Dergisi JO - KTD PB - Afyonkarahisar Sağlık Bilimleri Üniversitesi WT - DergiPark SN - 3061-9904 SP - 70 EP - 75 VL - 21 IS - 1 LA - en AB - Thiol/disulphide homeostasisis important in cancer. Studies are carried out to be biomarkers in cancerpatients. The aim of this study is to investigate the relationship betweenoxidative stress biomarker [thiol-disulphide homeostasis] and CEA [carcinoembryonicantigen], IMA [ischemic modified albumin], albumin, CA15-3 (cancer antigen 15-3) in patients with breast cancer and compare it withhealthy controls, which is conducted for the first time in the literature.A total of 80 participants including 39patients with breast cancer and 41 healthy individuals were included in thestudy. CEA, CA15-3, native thiol, total thiol, and disulphide as well asdisulphide/native thiol and disulphide/total thiol ratios were compared betweenthe groups. Native thiol[-SH], disulfide [-S-S] and total thiol [TT]concentrations were measured with a novel automated method. Patients participated in the study underchemotherapy. The study is a prospective study.In breast cancer group, IMA, albümin and CA-15-3 were obtained sitatiscally significantdifference compared to the controlegroup (p<0.05). Although in high CA-15-3 group decreasedSH/TT and increased SS/TT ratios, there wasn’t a significant change in theamount of thiol and disulfide. There was not anyrelationship between thiol-disulphide parameters and tumor markers in the thebreast cancer group.Itcould be said that changes in the thiol-disulphide homeostasismay notbe interact with CA 15-3 values. KW - Disulphide KW - thiol KW - tumor marker N2 - Tiyol/disülfit dengesi birçok hastalıkdaönemli bir oksidatif belirteçtir. Kanserli olgularda biyo-belirteç olması yönünde çalışmalaryapılmaktadır. Bu çalışmanın amacı meme kanserli hastalarda oksidatif stresbelirteci [tiyol-disülfid homeostazis] ile CA 15-3 (kanser antijeni 15-3) veCEA [karsinoembriyojenik antijen], IMA[iskemik modifiye albümin], albüminarasındaki ilişkiyi araştırmak ve literatürde ilk kez yapılan sağlıklıkontrollerle karşılaştırmaktır. Çalışmaya 39 kanserli hasta ve 41 sağlıklıtoplamda 80 hasta dahil edildi. Deneye katılan hastaların CEA, CA 15-3düzeyleri ve IMA, Albumin, Dinamik tiyol [-SH], Disülfit [-S-S] ve Total tiyol[TT] düzeyleri incelendi. Hastalar kemoterapi altında çalışmaya katıldılar.Çalışma prospektif bir çalışmadır. Kontrol -Meme CA grupları arasında IMA,albumin ve CA-15-3 açısından istatiksel olarak anlamlı fark olduğu belirlendi(p<0,05). CA 15-3 in yüksek olduğu grupta SH/TT değerlerinde azalma, SS/TTdeğerlerinde artış görülmesine rağmen tiyol ve disülfid miktarlarında anlamlıbir değişiklik olmadı. Tiyol-disülfid parametreleri ile Meme Ca grubunda tümörbiyo-belirteç değerleri arasında ilişki görülmedi. Tiyol-disülfidhomeostazisindeki değişikliklerin CA 15-3 ile etkileşmediği söylenebilir. CR - 1. Özdemir A, Erel O. Thıol-Dısulphıde Balance: Could be a new marker for Thyroid Cancer? Journal of Cancer Therapy [JCT]. 2018; 9: 8. CR - 2. Kamińska M,Ciszewski T, Łopacka-Szatan C, Miotła P, Starosławska E. Breast cancer risk factors. Prz Menopauzalny 2015; 14: 196–202. CR - 3. Robert G. Allen. Oxidative stress and superoxide dismutase in development, aging and gene regulation , Age [Omaha], 1998; 21[2]. CR - 4. Yalçın, GT. Flavonoidlerin kanser hücrelerine etkisi. Effect of Flavonoids on the Cancer Cells. phD Thesis, Institute Of Health Sciences, Selcuk University. Konya; 2013. CR - 5. Chianeh YR. Prabhu,K. Protein Thiols as an Indicator of Oxidative Stress, Archives Medical Review Journal.2014; 23[39]: 443-456. CR - 6. Cerutti PA. Prooxidant states and tumor promotion. Science. 1985; 227:375-81. CR - 7. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clinical Biochemistry.2014; 47[18]: 326-332. CR - 8. Hjerkind KV,Ellingjord-Dale M, Johansson AL. et al. Breast cancer risk factors and volumetric breast density in a national breast cancer screening program. Cancer Research. 2018; 78-13. CR - 9. Papadimitriou JP, Burchell JM, Plunkett, T, Graham R., Correa I, Miles D, Smith M. MUC1 and the Immunobiology of Cancer . Journal of Mammary Gland Biology and Neoplasia. 2002;7[2]: 209–221. CR - 10. Mohebtash M, Tsang KY, Madan RA.et al. A Pilot Study of MUC-1/CEA/TRICOM Poxviral-Based Vaccine in Patients with Metastatic Breast and Ovarian Cancer. Clin Cancer Res; 2011;17[22]; 1–10. CR - 11. Gürdöl F, Ademoglu, E.[Editors]. Biyokimya. 2. Baskı. Nobel Tıp Kitapevleri, 2010: 669. CR - 12. Gupta, RK, Patel AK, Shah N, et al. Oxidative Stress and Antioxidants in Disease and Cancer: A Review. Asian Pacific Journal of Cancer Prevention, 2014; l -15: 4405-4409. CR - 13. Ates I, Kaplan M, Yuksel M, et al. Determination of thiol/ disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 2016; 51:47-51. CR - 14. Kundi H, Ates I, Kızıltunc E, et al.A novel oxidative stress marker in acute myocardial infarction: thiol/disulphide homeostasis.Am J Emerg Med 2015; 33:1567-71. CR - 15. Özdemir A. Investıgatıon Of The Effects Of Mentha Spıcata Lamıaceae Mentha Extracts Prepared In Dıfferent Solvents Gıven To Rats Wıth Dry Powder On Blood, Β- Carotene, A,C Vıtamıns, Catalase, Glutathıone Peroxıdase, Glutathıone Reductase, Malondıaldehyde, Superoxıde Dısmutase Enzymes And Total Antı- Oxıdant Capacıty. phD Thesis,No:2010-003. Afyon Kocatepe University, Institute of Health Sciences.Departement of Biochemistery.Afyon;2010. CR - 16. Özdemir A, Sözbilir NB. The Effect of Mentha Spicata L. Dry Powder and its Different Typses Extracts on Certain Biochemical Parameters and Total Anti-oxidant Capacity. Afyon Kocatepe University Kocatepe Veterinary Journal, 2016; 9[1]:12-18. CR - 17. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions. 2006; 160 -1:40. CR - 18. Koçak S, Çelik L,Özbaş S, Dizbay Sak S, Tükün A, Yalçın B. Rısk Factors In Breast Cancer, Rısk Assessment And Preventıon:2010 Istanbul Consensus Meeetıng Report. The Journal of Breast Health 2011; 7: 2. CR - 19. Chianeh YR., Prabhu K. 2014, Protein Thiols as an Indicator of Oxidative Stress Oksidatif, Archives Medical Review Journal. 2014; 23[3] :443-456. CR - 20. Winther JR, Thorpe C. Quantification of thiols and disulfides.,Biochim Biophys Acta. 2014 Feb;1840[2]:838-46. CR - 21. Erel Ö. A new automated colorimetric method for measuring total oxidant status,Clinical Biochemistry. 2005; 38[12]: 1103–1111. CR - 22. Bektaş H, Vural G, Gümüşyayla Ş,Deniz O, Alışık M, Erel Ö. Akut İskemik İnmeli Hastalarda Tiyol-disülfid Dengesinin Araştırılması,Türk Beyin Damar Hast Dergisi.2015; 22 [1] :5. CR - 23. Dirican N, Dirican A, Şen O, Aynalı A, Atalay Ş, Bircan HA, Öztürk Ö, Erdoğan S, Çakır M, Akkaya A. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Rep. 2016;21[5]:197-203. CR - 24. Özkan S, Büyükdoğan M. Meme kanserinde prognostik faktörler: vakalarımızın retrospektif analizi, Tıp Araştırmaları Dergisi 8 [1] :9-14. CR - 25. Ergin M, Cendek BD, Neselioglu S, Avsar AF and Erel Ö. Dynamic thiol-disulfide homeostasis in Hyperemesis gravidarum. Journal of Perinatology [2015], 1–5. CR - 26. Kilinc F,Sener S, Akbas A, Neselioglu S, Erel Ö. and Aktas A. Investigation of Dynamic Thiol-Disulfide Homeostasis in Alopecia Areata Patients. British Journal of Medicine & Medical Research [2017], 21[6]: 1-7. CR - 27. Hazini A, Cemek M, Isıldak I, et al. Investigation of ischemia modified albumin, oxidant and antioxidant markers in acute myocardial infarction. Postepy Kardiol Interwencyjnej. 2015;11: 298–303. CR - 28. Eom JE, Lee E, Jeon KH, Sim J, Suh M, Jhon GJ, et al. Development of an albumin copper binding (ACuB) assay to detect ischemia modified albumin. Anal Sci 2014; 30:985-90. CR - 29. Beetham R, Monk C, Keating L, et al. Effects of storage at 20 degrees C on ischaemia-modified albumin results. Ann Clin Biochem. 2006;43:500–502. CR - 30. Yeh C-C, Hou MF, Tsai S-M et al. Superoxide anion radical, lipid peroxides and antioxidant status in the blood of patients with breast cancer. Clinica Chimica Acta, 2005;361(1–2): 104-111. CR - 31. Rajneesh C P, Manimaran A, Sasikala K R, Adaikappan P. Lipid peroxidation and antioxidant status in patients with breast cancer. Singapore Med J 2008; 49(8) : 640. UR - https://doi.org/10.18229/kocatepetip.560366 L1 - https://dergipark.org.tr/tr/download/article-file/1005208 ER -