TY - JOUR T1 - Farklı Benzil Amino Pürin ve Gibberellik Asit Kombinasyonunun Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Mikroçoğaltımı Üzerine Etkisi TT - The Effect of Different Combinations of Benzyl Amino Purine and Gibberellic Acid on Micropropagation of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne AU - Doğan, Muhammet PY - 2019 DA - August DO - 10.38001/ijlsb.562130 JF - International Journal of Life Sciences and Biotechnology JO - Int J. Life Sci. Biotechnol. PB - International Society of Academicians WT - DergiPark SN - 2651-4621 SP - 72 EP - 79 VL - 2 IS - 2 LA - tr AB - Rotala rotundifolia (Buch-Ham. ex Roxb)Koehne genenekseltıp sisteminde hastalıkların tedavisinde kullanılan önemli akuatik tıbbibitkidir. Bu çalışmada, 6-benzil amino purin (BAP) ve gibberellik asit (GA3)’infarklı kombinasyonlarını içeren kültür ortamında R. rotundifolia’nın boğum eksplnatlarından in vitro sürgün rejenerasyonu araştırılmıştır. Kültür ortamlarındailk sürgün oluşumları 10 gün sonunda gözlenmiştir. Sekiz hafta sonunda denemesonlandırılmış ve rejenerasyon verileri alınmıştır. Sürgün rejenerasyon oranı %72,22-100,00 arasında sıralanmıştır. %100sürgün rejenerasyon oranı 0,25-0,75 mg/L BAP + 0,25 mg/L GA3 içerenMS ortamında kaydedilmiştir. Eksplant başına maksimum sürgünler (18,38 adet) veen uzun sürgünler (2,36 cm) 0,25 mg/L BAP + 0,25 mg/L GA3 eklenmişkültürlerde elde edilmiştir. Çoğaltım ortamında sürgünlerin köklenmesinedeniyle ayrıca köklendirme çalışması yürütülmemiştir. Rejenere sürgünlerinakvaryum ortamına alıştırılması başarıyla sağlanmıştır. Bu rapor, tıbbi bitki R. rotundifolia’nın çoklu üretiminisunması bakımından önemlidir. İleride bu bitki ile yürütülecek sekondermetabolit üretimi ve gen aktarım çalışmalarına yardımcı olabilir. KW - Boğum eksplantı KW - Doku kültürü KW - Sürgün rejenerasyonu KW - BAP N2 - Rotala rotundifolia (Buch-Ham. ex Roxb)Koehne is an important aquatic medicinal plant for the treatment of diseases inthe traditional medicine system. In this study, in vitro shoot regeneration from thenodal explants of R. rotundifolia inculture medium containing different combinations of 6-benzyl amino purine (BAP)and gibberellic acid (GA3) was investigated. First shoot formationwas observed after 10 days in culture medium. At the end of eight weeks, thetrial was completed and regeneration data were obtained. Shoot regeneration rate is listed as72.22-100.00%. 100% shoot regeneration rate was recorded in MS medium including0.25-0.75 mg/L BAP + 0.25 mg/L GA3. The maximum shoots per explant (18.38) and thelongest shoots (2.36 cm) were obtained in cultures with 0.25 mg/L BAP + 0.25mg/L GA3. No rooting study was carried out due to the rooting of theshoots in the multiplication environment. 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