TY  - JOUR
T1  - Benzalkonyum klorürlerin Pseudomonas sp. BIOMIG1 tarafından farklı koşullar altında biyotransformasyon kinetiği
TT  - Biotransformation kinetics of benzalkonium chlorides by Pseudomonas sp. BIOMIG1 under different conditions
AU  - Yılmaz, Fatma Özge
AU  - Ertekin, Emine
AU  - Tezel, Ulaş
PY  - 2019
DA  - June
JF  - Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi
PB  - Pamukkale Üniversitesi
WT  - DergiPark
SN  - 2147-5881
SP  - 286
EP  - 291
VL  - 25
IS  - 3
LA  - tr
AB  - Dezenfektanaktif maddeleri kentsel ve endüstriyel atıksularda oldukça sık rastlanankirleticilerdir. Bu kirleticilerin biyolojik sistemlerde giderimi oldukçazordur. Yüzeysel sularda da sıkça karşılaşılan bu kirleticiler hem doğal hayatıhem de insan sağlığını tehdit etmektedir. Bu çalışmanın amacı, atıksuda en çok karşılaşılankirleticilerden biri olan benzalkonyum klorürlerin (BAK’ler) biyolojik sistemlerdeen verimli şekilde giderimini sağlayacak koşulların belirlenmesidir. Bu amaçla,atıksudan izole edilmiş BAK gideren bir bakteri olan Pseudomanas sp. BIOMIG1’infarklı koşullarda BAK biyotransformasyon kinetiği belirlenmiştir. Elde edilenveriler ve Michaelis-Menten modeli kullanılarak, bu mikroorganizmanın BAK biotransformasyonkinetiği parametreleri hesaplanmış ve uygulanan koşulların kinetiğe etkisibelirlenmiştir. BIOMIG1, BAK’leri 1.4 mg/L-saat hızında giderebilmekte ve bukirleticileri amonyak ve karbon dioksite dönüştürmektedir. Mililitrede yüz binadet bakteri yoğunluğu gibi düşük bakteri yoğunluklarında bile BAK gecikmeli deolsa yüksek hızda giderilebilmektedir. BAK homologlarının biyotransformasyonhızı karşılaştırıldığında, 14 karbon alkil zincir uzunluğuna sahip BAK enhızlı, 16 karbonlu BAK ise en yavaş biyotransformasyon hızına sahiptir. BAK giderimhızının en yüksek olduğu sıcaklık 35°, bu sıcaklık üstündeki sıcaklıklardaBIOMIG1 yaşayamamaktadır. Dolayısıyla yüksek sıcaklıklarda BAK parçalanması yabenzildimetilamin birikmesiyle sonlanmış ya da hiç gerçekleşmemiştir. Buçalışmanın sonuçları, özellikle BAK gibi mikrokirleticilerin arıtımınıhedefleyen ileri arıtma sistemlerinin tasarlanması ve işletilmesinde faydalıolacaktır.
KW  - Benzalkonyum klorür
KW  - Biyodönüşüm
KW  - Kinetik
KW  - Modelleme
KW  - Pseudomonas sp. BIOMIG1
N2  - Activeingredients of disinfectants are very common pollutants in urban and industrialwastewater. Removal of these contaminants is very difficult in biologicaltreatment systems. As a result; these pollutants, which are also frequentlydetected in surface waters, threaten both nature and human health. Theobjective of this study is to determine the optimum conditions that willprovide the most efficient removal of benzalkonium chlorides (BACs), a commoncontaminant, in biological treatment systems. For this purpose, BACbiotransformation kinetics were determined under different conditions usingPseudomanas sp. BIOMIG1, a bacterium that is the key BAC degrader in theenvironment. Using the data collected and the Michaelis-Menten growth model,BAC biotransformation kinetic parameters were calculated and the effect of theapplied conditions on kinetics was determined. BIOMIG1 can transform BACs at arate of 1.4 mg/L-hour and converts these pollutants into ammonia and carbondioxide. BAC mineralization can be achieved even at low bacterial densitiessuch as 100000 cells/mL after a short delay. When biotransformation rate of BAChomologs was compared, BAC with 14 carbon alkyl chain length had the fastestand BAC with 16 carbons had the slowest rate of biotransformation. Thetemperature at which the BAC biotransformation rate was the highest was 35degrees. BAC was converted to benzyldimethylamine at all temperatures above 35degrees since BIOMIG1 does not survive above this temperature. The outcomespresented in this study would be used for the design and operation of advancedtreatment systems targetting the removal of micropollutants like BACs.
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UR  - http://dergipark.org.tr/tr/pub/pajes/issue//583871
L1  - http://dergipark.org.tr/tr/download/article-file/747059
ER  -