@article{article_598852, title={DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS}, journal={Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, volume={8}, pages={191–202}, year={2019}, DOI={10.18036/estubtdc.598852}, author={Hürkan, Kaan and Ballı Yüksel, Merve and Kemeç Hürkan, Yasemin and Demir, Neslihan}, keywords={Salep orkideleri,Antioksidan aktivite,DPPH• analizi,Antimikrobiyal aktivite,Minimum inhibisyon konsantrasyonu}, abstract={<p class="AUBTDAabstracttitle"> <span lang="en-gb" xml:lang="en-gb">ABSTRACT </span> </p> <p class="AUBTDAabstract"> <b> <span style="font-size:9pt;font-family:’Times New Roman’, serif;" lang="en-gb" xml:lang="en-gb">  </span> </b> <b>I </b> <span lang="en-gb" xml:lang="en-gb">n
this study we evaluated the secondary metabolites, total phenolic (TPC) and
flavonoid contents (TFC), antioxidant and antimicrobial activities of salep
orchids, <i>Anacamptis morio </i>, <i>Anacamptis pyramidalis </i>, <i>Neotinea tridentata </i>, <i>Ophrys mammosa </i>, <i>Ophrys lutea </i>, and <i>Ophrys
speculum </i>. DPPH free radical scavenging assay was used to determine the
antioxidant activities of <i>n </i>-hexane,
chloroform, methanol and water extracts of the plants. The antimicrobial
activities were also determined by the Broth micro-dilution method. The
extracts were studied for antimicrobial activity by the Minimum Inhibitory
Concentration (MIC) approach against seven clinical pathogenic bacteria and two
fungi. Phytochemical screening revealed that the presences of coumarins,
flavonoids, flavanones, cardiac glycosides, proteins and quinones. The extracts
had variable TPC and TFC, with values of 4.46 ± 0.19–45.83 ± 1.86 mg gallic
acid equivalent/g dry weight and 0.67 ± 0.04–8.64 ± 0.37 mg quercetin
equivalent/g dry weight respectively. <i>O.
speculum </i> had the highest (35.12%) antioxidant activity, followed by <i>O. mammosa </i> (33.17%). Chloroform extracts
of all species showed significant antioxidant and antimicrobial activity. These
bioactivities of the chloroform extracts were positively associated with the
total phenolic and flavonoid contents. The MIC concentrations ranged from
0.156–20 mg/mL. The present investigation shows that the extracts of these
species, especially chloroform extracts, could be used as potential antioxidant
and antimicrobial sources. </span> </p> <p class="MsoNormal" style="margin-bottom:.0001pt;line-height:normal;"> <span style="font-size:9pt;font-family:’Times New Roman’, serif;" lang="en-gb" xml:lang="en-gb">  </span> </p>}, number={2}, publisher={Eskişehir Teknik Üniversitesi}