@article{article_604775, title={Effects of Short-term High Glucose on NIH/3T3 Fibroblast Proliferation, Apoptosis, and Collagen Type I Production}, journal={Tıp Fakültesi Klinikleri Dergisi}, volume={2}, pages={91–95}, year={2019}, author={Soydaş, Tuğba and Yaprak Saraç, Elif and Çınar, Suzan and Yenmiş, Güven and Doğan, Sibel and Solakoğlu, Seyhun and Tunçdemir, Matem and Kanıgür Sultuybek, Gönül}, keywords={High glucose, Collagen, NIH/3T3 fibroblast, Apoptosis}, abstract={<p>Objective: To investigate the mechanisms of in vitro high glucose induced model of liver <br />fibrosis. <br />Material and methods: The effects of high glucose concentration on fibroblast proliferation <br />were investigated by BrdU immunostaining. Apoptosis and necrosis levels were analyzed by <br />flow cytometric assay. The content of collagen type I was measured by Collagen Estimation <br />Assay through ELISA. <br />Results: A high glucose medium not only increased NIH/3T3 fibroblast proliferation, but also <br />increased type I collagen synthesis, showing a similar condition to the fibrosis. Moreover, the <br />high glucose caused an increased level of cellular apoptosis and necrosis. <br />Conclusions: High glucose modulates the fibrosis in NIH/3T3 fibroblast cells via inducing the <br />production of type I collagen while maintains the homeostasis by inducing the apoptosis and <br />necrosis of cells. <br /> </p>}, number={3}, publisher={İstanbul Aydın Üniversitesi}