TY - JOUR T1 - Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi TT - Effect of aspirin on protein levels of paraoxonase enzymes and arylesterase activity in human-derived hepatoma cells AU - Özgün, Eray AU - Sayılan Özgün, Gülben PY - 2019 DA - September DO - 10.19161/etd.610836 JF - Ege Tıp Dergisi JO - EJM PB - Ege Üniversitesi WT - DergiPark SN - 1016-9113 SP - 295 EP - 302 VL - 58 IS - 3 LA - tr AB - Amaç: Bu çalışmanın amacı, aspirinininsan kaynaklı hepatoma hücrelerinde paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3protein düzeylerine ve arilesteraz aktivitesine etkisini araştırmaktır.Gereç ve Yöntem: HepG2hücreleri kontrol, 0.25 mM aspirin, 0.5 mM aspirin ve 1 mM aspirin olmak üzere4 gruba ayrıldı ve hücreler 48 saat boyunca sırasıyla 0, 0.25, 0.5 ve 1 mMaspirin ile inkübe edildi. Hücre canlılığı 3-(4,5-Dimetil-2-tiazolil)-2,5-difenil-2H-tetrazoliumbromür testi ile ölçüldü. Paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 proteindüzeyleri western blot yöntemiyle ölçüldü. Arilesteraz aktivitesi substratolarak fenilasetat kullanılarak spektrofotometrik olarak ölçüldü.Bulgular: 0.5 mM ve 1 mM aspirinhücre canlılığında anlamlı azalmaya yol açtı. Aspirin konsantrasyonlarıparaoksonaz-1 ve paraoksonaz-2 protein düzeylerini anlamlı olarak değiştirmedi.0.5 ve 1 mM aspirin paraoksonaz-3 protein düzeylerini anlamlı olarak arttırdı.0.25 mM, 0.5 mM ve 1 mM aspirin, arilesteraz aktivitesini anlamlı olarakarttırdı.Sonuç: Çalışmamız aspirinininsan kaynaklı hepatoma hücrelerinde paraoksonaz-1 ve paraoksonaz-2 proteindüzeylerini değiştirmediğini, paraoksonaz-3 protein düzeylerini ve arilesterazaktivitesini arttırdığını gösterdi. KW - Aspirin KW - paraoksonaz-1 KW - paraoksonaz-2 KW - paraoksonaz-3 KW - arilesteraz. N2 - Aim: The aim of this study is to investigate the effect of aspirin onparaoxonase-1, paraoxonase-2 and paraoxonase-3 protein levels and arylesteraseactivity in human-derived hepatoma cells.Materials and Methods: HepG2 cells were divided into four groups: control,0.25 mM aspirin, 0.5 mM aspirin and 1 mM aspirin and cells were incubated with0, 0.25, 0.5 and 1 mM aspirin, respectively. Cell viability was evaluated by3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay.Paraoxonase-1, paraoxonase-2 and paraoxonase-3 protein levels were measured bywestern blotting. Arylesterase activity was measured spectrophotometrically byusing phenylacetate as substrate.Results: 0.5 mM and 1 mM aspirin caused a significant decrease on cell viability.Aspirin concentrations did not significantly change paraoxonase-1 andparaoxonase-2 protein levels. 0.5 mM and 1 mM aspirin significantly increasedparaoxonase-3 protein levels. 0.25 mM, 0.5 mM and 1 mM aspirin significantlyincreased arylesterase activity.Conclusion: Our study showed thataspirin does not change paraoxonase-1 and paraoxonase-2 protein levels but increasesparaoxonase-3 protein levels and arylesterase activity in human-derivedhepatoma cells CR - Gaglia MA Jr, Clavijo L. Cardiovascular pharmacology core reviews: aspirin. J Cardiovasc Pharmacol Ther 2013; 18 (6): 505-13. CR - Schrör K, Rauch BH. Aspirin and lipid mediators in the cardiovascular system. Prostaglandins Other Lipid Mediat 2015; 121 (Pt A): 17-23. CR - She ZG, Chen HZ, Yan Y, Li H, Liu DP. 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