@article{article_616173, title={CYTOTOXICITY OF VENOM FROM ENDOPARASITOID PIMPLA TURIONELLAE L. (HYMENOPTERA: ICHNEUMONIDAE) ON GLIOBLASTOMA CELLS}, journal={Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, volume={9}, pages={219–225}, year={2020}, DOI={10.18036/estubtdc.616173}, author={Çim, Selin and Altuntaş, Hülya and Ak, Ayşe}, keywords={Pimpla turionellae, endoparazitoid venom, glioblastoma, sitotoksisite, MTT, MTT}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;line-height:normal;"> <span lang="en-us" style="font-size:9pt;font-family:’Times New Roman’, serif;" xml:lang="en-us">In the treatment of brain
cancer, the inhibition of cancer cell proliferation using anti-cancer agents is
a priority approach. Venom of endoparasitoid wasps also may be a candidate for
the development of new therapeutic agents. In this context, endoparasitoid <i>Pimpla turionellae </i> L. (Hymenoptera:
Ichneumonidae) venom may have an anti-carcinogenic effect on glioblastoma that
resists traditional therapies by various mechanisms. Therefore, we aimed to
investigate endoparasitoid venom cytotoxicity on glioblastoma cells. For this
purpose, the cytotoxic potential of venom from <i>P. turionellae </i> was evaluated on rat C6 glioblastoma cell lines with
methyl thiazolyl tetrazolium assay (MTT assay). <i>P. turionellae </i> venom was isolated from 15±2 day-old females.
Different concentrations of <i>P.
turionellae </i> venom (176.6-1.83 μg/ml) were applied to C6 rat glioblastoma
cells in vitro. Results of MTT assay showed that the viability of C6 cells in
vitro significantly decreased depending on the parasitoid venom concentrations.
Therefore, <i>P. turionellae </i> venom
showed cytotoxic activity in a time- and dose-dependent manner. In conclusion,
the results from this research could be used as primary data of venom
cytotoxicity for investigation of new chemotherapeutic agents against malignant
tumors. </span> </p> <p> </p>}, number={2}, publisher={Eskişehir Teknik Üniversitesi}, organization={Eskisehir Technical University, Scientific Research Projects Commission}