TY - JOUR T1 - Chemical Vapor Deposition of Poly(hydroxyethyl methacrylate-glycidyl methacrylate) Thin Film Coatings for Immobilization of Human Serum Albumin AU - Sarıipek, Fatma AU - Çağıl, Esra AU - Karaman, Mustafa PY - 2019 DA - December JF - Uluslararası Çevresel Eğilimler Dergisi JO - IJENT PB - Muhammed Kamil ÖDEN WT - DergiPark SN - 2602-4160 SP - 96 EP - 119 VL - 3 IS - 2 LA - en AB - In this study, poly(2-hydroxyethyl methacrylate-glycidylmethacrylate) (P(HEMA-GMA) thin films were deposited on crosslinked polyvinylalcohol (PVA) supports by initiated chemical vapor deposition (iCVD). Use ofthe tert-butyl peroxide as an initiator allowed very high deposition rates upto 125 nm/min at a filament temperature of 280 oC. FTIR and XPSanalyses of the deposited films confirmed that the epoxide functionality of thecopolymers increased with increasing glycidyl methacrylate fraction in the reactorinlet. The potential of the glycidyl methacrylate containing copolymer films toact as substrates for protein immobilization was revealed. The immobilizationof Human Serum Albumin (HSA), which is the main protein to carry fatty acidsand metals to target tissues, was carried out via solid phase extraction. Theeffects of film composition and thickness on binding capacities of the proteinto the polymers were studied. The maximum protein binding for the iCVDsynthesized copolymer films was found to be 223 µg.cm-2. Proteinbinding was also clarified by FTIR, AFM, and SEM analyses. The mildimmobilization conditions, easy and rapid membrane preparation, one-stepprotein binding at substantially higher levels and membrane reusability makeiCVD deposited P(HEMA-GMA) films usefulfor biomolecules immobilization and for several biochemical processes. KW - iCVD KW - Thin film hydrogel membrane KW - Protein immobilization KW - P(HEMA-GMA) KW - Crosslinked PVA CR - 1. 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