@article{article_622902, title={Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water}, journal={Acta Aquatica Turcica}, volume={16}, pages={170–178}, year={2020}, DOI={10.22392/actaquatr.622902}, author={Mercimek Takcı, Hatice Aysun and Bakırhan, Pemra and Genç, Sema and Kaptanoğlu, Melike}, keywords={Biyofilm,GSBL,metallo-beta laktamaz,SEM}, abstract={<p class="MsoNormal" style="margin-bottom:.0001pt;text-align:justify;text-indent:14.2pt;line-height:normal;"> <span style="font-family:’Times New Roman’, serif;">The production extended spectrum beta lactamase (ESBL) and metallo-beta lactamase </span> <span style="font-family:’Times New Roman’, serif;"> of strains grown biofilm that were isolated from Kilis State Hospital sewage water were investigated in this study. Congo red agar, tube adherence, tissue culture plate tests and SEM analysis were performed for the detection of biofilm production. </span> <span style="font-family:’Times New Roman’, serif;">The </span> <span style="font-family:’Times New Roman’, serif;">extended spectrum beta lactamase and metallo-beta lactamase expression of biofilm producing bacteria was tested by using </span> <span style="font-family:’Times New Roman’, serif;">Kirby-Bauer disc diffusion method according to CLSI guidelines. 5 clinical isolates were revealed to be biofilm producer. From of these isolates, </span> <em>Enterococcus </em> <em> <span style="font-style:normal;"> spp. (C2) and </span> </em> <i> <span style="font-family:’Times New Roman’, serif;">E. coli </span> </i> <span style="font-family:’Times New Roman’, serif;">strains showed </span> <span style="font-family:’Times New Roman’, serif;">strong biofilm producing. One of <i>Enterococcus </i> isolates, SEM micrograph of C1 was viewed the weak adherence on glass surface. </span> <span style="font-family:’Times New Roman’, serif;"> Only one from 5 isolates, </span> <em>Enterococcus </em> <em> <span style="font-style:normal;"> spp. (C2) strain was determined to synthesis ESBL enzyme. None of strains was observed to not produce </span> </em> <span style="font-family:’Times New Roman’, serif;">metallo-beta lactamase. ESBL and biofilm production provide an important pathogenic character allowing protection against antibiotic treatments to <i>Enterococcus </i> spp. (C2). <b> </b> </span> <span style="font-family:’Times New Roman’, serif;"> </span> </p> <p> </p>}, number={2}, publisher={Isparta Uygulamalı Bilimler Üniversitesi}