TY - JOUR T1 - Azot Kaynakları ve Konsantrasyonlarının Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae)'nın Büyüme ve Pigment Komposizyonuna Etkisi. TT - Effects of nitrogen sources and concentration on the growth and pigment composition of the Nannochloropsis oculata (Droop, 1955) (Eustigmatophyceae). AU - Durmaz, Yaşar PY - 2006 DA - September JF - Ege Journal of Fisheries and Aquatic Sciences JO - EgeJFAS PB - Ege Üniversitesi WT - DergiPark SN - 2148-3140 EP - 1 VL - 23 IS - 3 LA - tr AB - Azot kaynakları ve konsantrasyonlarının Nannochloropsis oculata türünün karotenoidler ve klorofil a üretimi üzerine etkileri incelendi. N. oculata, laboratuvarda 2 L’lik erlenlerde, F/2 besin ortamı ile zenginleştirilmiş steril deniz suyunda yığın kültürleri yapıldı (Tuzluluk % 2.5). Kültürler 18±1ºC sabit sıcaklıkta tutuldu. Azot kaynakları olarak sodyum nitrat, amonyum klorür ve üre olarak kullanıldı. Azot kaynakları besin ortamlarına 882 ve 441 µmol L-1 konsantrasyonlar kullanıldı. Denemeler sonunda β-Karoten, klorofil-a, klorofil-b, violaksantin, kantaksantin, astaksantin, vokeriyaksantin ve zeaksantin N.oculata’da ana pigmentler olarak TLC yöntemi ile saptandı. Toplam karotenoidler ve klorofil a düzeyleri bakımından en yüksek değerler 882 µmol L-1 NO3- -N ile hazırlanan besin ortamında ilk alınan örneklerde elde edildi (değerler sırasıyla, 4.17±0.37 mg g-1 dw ve 12.42±1.08 mg gdw ). N. oculata türü için inorganik azot kaynaklarından NO-N ve NH4+-N ile büyüme ve biyokimyasal yapısı açısından benzer sonuçlar elde edildi. Fakat üre-N’nun bu tür için uygun kaynak olmadığı tespit edildi KW - Mikroalgler KW - Nannochloropsis oculata KW - karoten KW - β-karoten KW - klorofil a ve TLC CR - Brown, M.R., S.W. Jeffrey, C.D. Garland, (1989), Nutritional aspects of microalgae used in mariculture: a literature review. CSIRO Mar. Lab. Rep. 205, 44. CR - Fidalgo, J.P., A. Cid, J. Abalde, C. 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