@article{article_700189, title={Insecticidal activities of wild type and recombinant invertebrate iridescent viruses on five common pests}, journal={Turkish Journal of Entomology}, volume={44}, pages={365–373}, year={2020}, DOI={10.16970/entoted.700189}, author={Gencer, Donus and Yesilyurt, Aydin and Güllü, Mustafa and Demir, İsmail and Nalcacıoglu, Remziye}, keywords={Omurgasız iridesan virüs, insektisidal aktivite, rekombinant virüs}, abstract={Invertebrate iridescent virus 6 (IIV6) can infect a broad range of pest insect species. Viruses with new features created by recombinant DNA technology can be used effectively as biological control agents. Previously, recombinants have been constructed: IIVs harboring green fluorescent protein gene (gfp) in place of IIV6 157L ORF (rCIV-Δ157L-gfp) and a scorpion <i>Androctonus australis </i> (Linnaeus, 1758) insect toxin gene (AaIT) fused with gfp (rCIV-Δ157L/gfp-AaIT). In this study, wild type IIV6 and the two recombinants, were evaluated for their ability to cause infections on <i>Helicoverpa armigera </i> (Hübner, 1805) (Lepidoptera: Noctuidae), <i>Spodoptera littoralis </i> (Boisduval, 1883) (Lepidoptera: Noctuidae), <i>Lymantria dispar </i> (Linnaeus, 1758) (Lepidoptera: Erebidae), <i>Euproctis chrysorrhoea </i>(Linnaeus, 1758) (Lepidoptera: Erebidae) and <i>Tenebrio molitor </i> (Linnaeus, 1758) (Coleoptera: ‎Tenebrionidae) larvae. This study was performed at Karadeniz Technical University, Department of Biology during 2018 and 2019. Five different concentrations (103, 104, 105, 106 and 107 TCID50/ml) of viruses were used to inject each insect larvae. All larvae, infected with rCIV-Δ157L/gfp-AaIT, became paralyzed, except S. littoralis. The LC50 of insect larvae infected by rCIV-Δ157L/gfp-AaIT were determined as 0.3 x 107, 0.7 x 105, 0.2 x 105, 0.15 x 105, 0.7 x 104 TCID50/ml on <i>S. littoralis </i>, <i>T. molitor </i>, <i>L. dispar </i>, <i>H. armigera </i>and <i> E. chrysorrhoea </i>, respectively. LT50 values, calculated according to the highest virus concentrations, were found as 10.5, 6.2, 4.7, 7.5 and 5 d on <i>S. littoralis </i>, <i>T. molitor </i>, <i>L. dispar </i>, <i>H. armigera </i>and <i>E. chrysorrhoea </i>, respectively, for rCIV-Δ157L/gfp-AaIT. This study showed that recombinant IIV6 has increased pathogenicity on some insects from Lepidoptera and Coleoptera. <br />}, number={3}, publisher={Türkiye Entomoloji Derneği}