@article{article_705435, title={CD44 EXPRESSION IN PROSTATIC ADENOCARCINOMA: CORRELATION WITH GLEASON GRADES}, journal={The Journal of Kırıkkale University Faculty of Medicine}, volume={22}, pages={233–239}, year={2020}, author={Balcı, Mahi and Özdemir, Gülhan}, keywords={Prostat karsinogenezis, Kök Hücreler, Prostatik Hiperplazi, Keratinler}, abstract={<div style="text-align:justify;"> <span style="font-size:14px;"> <b>Objective: </b>CD44, a cell-surface adhesion protein, is a marker of stem cells and is involved in the structural maintenance of prostate gland basal cells. It plays an important role in prostate carcinogenesis; however, research results till date have been inconsistent. The current study was conducted to evaluate the expression level of CD44 among carcinomas with different Gleason growth patterns and hyperplastic glands. </span> </div> <span style="font-size:14px;"> </span> <div style="text-align:justify;"> <b>Material and Methods: </b> Eighty archival tissue samples from patients with either prostate carcinoma of differing Gleason patterns or benign prostate hyperplasia from the pathology archives of the Kırıkkale University, Faculty of Medicine, from 2011 to 2018 were included. Immunoreaction to CD44 antibodies was evaluated by calculating positively stained cell percentage and staining intensity. Mean values and significance were determined using the Kruskal–Wallis test. p<0.05 was considered significant. </div> <span style="font-size:14px;"> </span> <div style="text-align:justify;"> <b>Results: </b> CD44 staining was more diffuse and intense in most of benign hyperplastic tissues compared to carcinoma cases (p values ranged from <0.01 to 0.005). Expression level gradually decreased with increasing severity of histologic pattern. Pattern 3 tumor showed higher percentage of positively stained cells than pattern 4 (p<0.01) There was no positivity with CD44 in grade 5 tumours. Also, in pattern 4 only few cells reacted with CD44. There was no statistically significant difference in the staining score between pattern 4 and 5 (p=0.278). </div> <span style="font-size:14px;"> </span> <div style="text-align:justify;"> <b>Conclusion: </b> We conclude that once cancerous properties have been established, cells tend to lose the ability of CD44 expression. CD 44 expression in low grade carcinomas suggest that CD 44 maintains the tumor in a differentiated, gland forming state so it may not act as a cancer stem cell marker in prostate carcinogenesis. </div> <p> </p>}, number={2}, publisher={Kırıkkale Üniversitesi}