@article{article_709504, title={Comparing Autosomal SSR and PCR-RFLP Markers to Determine Phylogenetic Relationship Based on Genetic Distances in Livestock}, journal={Journal of Animal Production}, volume={61}, pages={135–141}, year={2020}, DOI={10.29185/hayuretim.709504}, author={Karslı, Taki and Demir, Eymen and Argun Karslı, Bahar and Fidan, Hüseyin Göktuğ and Balcıoğlu, Murat}, keywords={Çiftlik hayvanları, UPGMA dendogramı, moleküler markerler, filogeni}, abstract={<div style="text-align:justify;"> <span style="font-size:14px;">Objective: Many molecular tools are available to analyse phylogenetic relationships  </span> <span style="font-size:14px;">in livestock. Nowadays, Simple Sequence Repeats and Single Nucleotide  </span> <span style="font-size:14px;">Polymorphisms are commonly used molecular techniques to determine  </span> <span style="font-size:14px;">phylogenetic relationships in livestock breeds or types. However, alternative  </span> <span style="font-size:14px;">molecular techniques may be preferred to conduct phylogenetic analysis in case of  </span> <span style="font-size:14px;">limiting conditions such as budget and time. In this context, in the present study,  </span> <span style="font-size:14px;">Simple Sequence Repeats and Polymerase Chain Reaction- Restriction Fragment  </span> <span style="font-size:14px;">Length Polymorphism techniques were compared to reveal phylogenetic  </span> <span style="font-size:14px;">relationship based on genetic distances. </span> </div> <div style="text-align:justify;"> <span style="font-size:14px;"> <br /> </span> </div> <span style="font-size:14px;"> </span> <div style="text-align:justify;"> <span style="font-size:14px;">Material and Methods: In this study, 11 different layer pure chicken lines represented by 30 individuals for each line were genotyped based on 11 Polymerase Chain Reaction- Restriction Fragment Length Polymorphism and 17 Simple Sequence Repeats loci to analyse phylogenetic relationship. </span> </div> <div style="text-align:justify;"> <br /> </div> <span style="font-size:14px;"> </span> <div style="text-align:justify;"> <span style="font-size:14px;">Results: Both techniques showed almost similar results in terms of Unweighted Pair Group Method with Arithmetic Mean dendrogram created based on genetic distances. White and brown chicken lines were separated by both Polymerase Chain Reaction- Restriction Fragment Length Polymorphism and Simple Sequence Repeats techniques in harmony with their genetic origins and breeding history. </span> </div> <div style="text-align:justify;"> <br /> </div> <span style="font-size:14px;"> </span> <div style="text-align:justify;"> <span style="font-size:14px;">Conclusion: It is suggested that Polymerase Chain Reaction- Restriction Fragment Length Polymorphism technique may be preferred to analyse phylogenetic relationship based on genetic distance, when the budget, time and laboratory infrastructure are limited.  </span> </div>}, number={2}, publisher={Ege Zootekni Derneği}, organization={The Scientific Research Projects Coordination Unit of Akdeniz University}