@article{article_72667, title={Assesment of PTCH1 promoter methylation in BCC carcinogenesis}, journal={EASTERN JOURNAL OF MEDICINE}, volume={19}, pages={105–106}, year={2014}, author={Sayın Kocakap, Derya}, keywords={Basal Cell Carcinoma, PTCH1, methylation, COBRA}, abstract={<p>Basal Cell Carcinoma (BCC) is the most common cancer in humans. It is known that <em>PTCH1 </em> mutations in Sonic Hedgehog pathway play significant role in BCC  pathogenesis. However  <em>PTCH1 (Patched) </em> inactivation through promoter methylation is still under investigation. In this study promoter methylation of the <em>PTCH1 </em> <strong> </strong>gene <strong> </strong>was analysed <strong> </strong>by <strong> </strong>Combined Bisulphite Restriction Analysis in 10 formalin-fixed paraffin-embedded (FFPE) BCC tissues. There was no methylation in any of the samples. Results suggest that <em>PTCH1 </em> promoter methylation is not effective in BCC carcinogenesis and FFPE tissues are not suitable for methylation analysis since 44 specimens were included in the study and only 10 of them gave result. </p>}, number={2}, publisher={Van Yüzüncü Yıl Üniversitesi}