@article{article_759607, title={In vitro antimicrobial, antioxidant and anti-inflammatory evaluation of Eucalyptus globulus essential oil}, journal={Natural Volatiles and Essential Oils}, volume={7}, pages={1–11}, year={2020}, author={Göger, Gamze and Karaca, Nursenem and Büyükkılıç, Betül and Demirci, Betül and Demirci, Fatih}, keywords={: Antibacterial, anti-inflammatory, Eucalyptus globulus, Propionibacterium acnes, Staphylococcus}, abstract={Eradication of Propionibacterium acnes and associated skin pathogenic species such as Staphylococcus aureus and S. epidermidis <br />involve anti-oxidant as well as anti-inflammatory effects besides antimicrobial action. For this purpose, Pharmacopoeia Grade (PhEur) <br />Eucalyptus globulus essential oil was evaluated against the human pathogenic species such as P. acnes ATCC 6919, P. acnes ATCC <br />11827, S. aureus ATCC 6538 and S. epidermidis ATCC 12228 using an in vitro microdilution method. The composition and quality of <br />the essential oil was confirmed both by GC/FID and GC/MS techniques, respectively. The in vitro radical-scavenging activity was <br />evaluated using the photometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay; the anti-inflammatory activity assay performed <br />by using the in vitro lipoxygenase (5-LOX) enzyme inhibition assay. Essential oil analysis confirmed the presence of 1,8-cineole (80.2 <br />%), p-cymene (6.6 %), and limonene (5 %) as main components. The antibacterial performance of the tested oil was more susceptible <br />against Staphylococcus species (MIC=625 µg/mL) compared to P. acnes (MIC=1250 µg/mL). 5-LOX inhibitory activity was determined <br />as IC50 = 58 ±1,4 µg/mL for the essential oil, compared to the inhibition of the standard nordihydroguaiaretic acid = NDGA. The <br />preliminary experimental results suggest that the Eucalyptus essential oil and its major constituent 1,8-cineole acts against skin <br />pathogenic bacteria as a mild natural antimicrobial with anti-inflammatory effects, for further potential topical applications. }, number={3}, publisher={Badebio Biotechnology Ltd.}, organization={Anadolu Üniversite Bilimsel Araştırma Projeleri Birimi}