@article{article_779956, title={PCR-RFLP analyses of Aspergillus spp. Isolated from Pistachio in Turkey}, journal={The Journal of Turkish Phytopathology}, volume={49}, pages={25–33}, year={2020}, author={Alaayadi, Ali and Ali, Safa and Başbuğa, Selçuk and Atik, Omar and Can, Canan}, keywords={Antep Fıstığı, Aspergillus spp, PCR-RFLP}, abstract={Pistachio (Pistacia vera L.) is a strategic crop plant and Turkey is one of the major pistachio producing county together with Iran, Syria and the United States of America. Pistachio trees are affected by many fungal diseases. The Aspergillus spp. are one of the most important agent that produce mycotoxins and also known as human and animal pathogens. This study was conducted to determine the genomic DNA polymorphisms from Aspergillus spp. which were isolated from pistachio fruits collected from different climatic zones in Turkey. Total of 27 Aspergillus spp. isolates were used and specific primers for ITS, Actin and β-tubulin genes were subjected to RFLP analysis by using Hind III, Pst I, Taq I and Eco RI restriction enzymes. Three different groups were defined with the enzyme Taq I in the β-tubulin region. The results revealed low polymorphism and genetic diversity in Aspergillus spp. with PCR-RFLP of the ITS and actin regions.}, number={2}, publisher={Türkiye Fitopatoloji Derneği}