@article{article_806050, title={Association of BRCA1 (G22231T, T25025A, C28300A) polymorphisms with subclinical mastitis and milk yields in Holstein Cattle}, journal={Harran University Journal of the Faculty of Veterinary Medicine}, volume={10}, pages={12–19}, year={2021}, DOI={10.31196/huvfd.806050}, author={Daldaban, Fadime and Arslan, Korhan and Akçay, Aytaç and Sohel, Md Mahmodul Hasan and Akyüz, Bilal}, keywords={BRCA1 gene, Holstein, Mastitis, PCR-RFLP}, abstract={In this study, it was aimed to investigate the relationship between three SNP on the BRCA1 gene and subclinical mastitis, as well as milk yields during four lactation periods in Holstein breed cattle. The animal material of the study consisted of 151 Holstein cows reared under the same care and feeding conditions. Complete DNA was isolated from blood samples by using phenol: chloroform: isoamyl alcohol method. Genotyping of G22231T, T25025A and C28300A SNPs on the BRCA1 was done by the PCR-RFLP method. Subclinical mastitis of animals was determined in milk samples by California Mastitis Test (CMT). In the study, CMT data, four lactation periods’ milk yields, and proportional distributions of SNP genotypes were calculated. Statistical significance of the data was determined by the Pearson chi-square test. The relationship between C28300A SNP, which was monomorphic, and subclinical mastitis has not been analyzed. In the samples examined, the difference between the proportional distribution of SNP genotypes of G22231T and T25025A in subclinical mastitis and healthy groups was not found statistically significant (P>0.05). As a result of the Chi-square (χ2) analysis conducted, it was observed that the Holstein cattle were in the Hardy-Weinberg equilibrium in terms of G22231T and T25025A SNPs. Considering the physiological processes in which the BRCA1 gene is involved, it was thought that these genes and SNPs should be evaluated with more samples in studies on resistance to mastitis.}, number={1}, publisher={Harran Üniversitesi}