@article{article_80743, title={EFFECT OF SOME DRUGS ON SHEEP LIVER CARBONIC ANHYDRASE I AND II}, journal={Erzincan University Journal of Science and Technology}, volume={8}, pages={201–212}, year={2015}, DOI={10.18185/eufbed.22429}, author={Çankaya, Murat and Kuzucu, Mehmet}, keywords={Karaciğer, Antibiyotik, İnhibisyon}, abstract={<p>Effects of clindamycin, dexketoprofen trometamol, Benzylpenicillin, (penicillin G), Phenoxymethylpenicillin (penicillin V) were examined on purified sheep carbonic anhydrase I and II (SCAI, SCA-II). In our study, we were chosen sheep liver tissue; because the liver is responsible for detoxification of xenobiotics. The CA isoenzyme have a very important duties on organisms, the effect of xenobiotics on the CA enzyme was reported. We exhibited the inhibition effects of these drugs against two sheep liver carbonic anhydrase isozymes (SCA I and SCA II). SCA I and SCA II isozymes were purified with about a specific activity  of 1108,51 EU/mg and 5706,67 EU/mg,  120,62 and  620,96 purification fold by using Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Purity of the SCA I and SCA II isoenzyme was checked by SDS-PAGE technique and subunit molecular masses of the SCA I was found as 29 kDa and SCA II found as 31 kDa. The usage of clindamycin, dexketoprofen trometamol, Benzylpenicillin, (penicillin G), Phenoxymethylpenicillin (penicillin V) in sheep liver especially during necrotic hepatitis disease is important for scientific researches. As most of the antibiotic and antihistaminic can be dangerous for sheep liver. Also, it is important to study the inhibition effects of these drugs on SCA I and II izoenzymes. <strong> </strong> </p>}, number={2}, publisher={Erzincan Binali Yıldırım Üniversitesi}