TY - JOUR T1 - Sıcaklığa Dirençli Alfa-amilaz Enzimi Üreten Mezofilik Bakteri İzolasyonu ve Enzimin Kısmi Karakterizasyonu TT - Isolation of Thermostable Alpha-amylase Producing Mesophilic Bacterium and Partial Characterization of the Enzyme AU - Özcan, B. Devrim AU - Özdemir, Gültekin PY - 2020 DA - December Y2 - 2020 DO - 10.47495/okufbed.824607 JF - Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi JO - Osmaniye Korkut Ata University Journal of The Institute of Science and Techno PB - Osmaniye Korkut Ata Üniversitesi WT - DergiPark SN - 2687-3729 SP - 146 EP - 153 VL - 3 IS - 2 LA - tr AB - Bu çalışmada, Niğde ili Ulukışla ilçesi Çiftehan bölgesi sınırları içinde bulunan kaplıcadan alınan toprak örneklerinden α-amilaz aktivitesine sahip Bacillus izolasyonu yapılmıştır. İzolat bakteri, Bacillus sp. GA4 olarak isimlendirilmiştir. Bacillus sp. GA4 izolatına ait α-amilaz enziminin optimum pH ve sıcaklık değerleri sırasıyla 6.0-8.0 ve 50 ºC olarak bulunmuştur. Enzim 40 ºC’de 30 dk. ön inkübasyon sonrasında aktivitesini tamamen korumuştur. Enzim, 50 ºC ve 60 ºC’de 30 dk. ön inkübasyon sonrasında ise sırasıyla %21 ve %37’lik aktivite kaybına uğramıştır. MgCl2 ve FeSO4 enzim aktivitesini sırasıyla %13 ve %12 seviyelerinde artırırken, CuSO4, CaCl2 ve EDTA sırasıyla %81, %38 ve %33 oranlarında inhibe etmiştir. Bacillus sp. GA4 α-amilaz enziminin moleküler ağırlığı SDS-PAGE zimogram analizi ile yaklaşık olarak 55 kDa bulunmuştur. İzolatın CFX, CN, TE, RD, S, Amp ve P antibiyotiklerine karşı hassas, SH antibiyotiğine ise dirençli olduğu gözlenmiştir. KW - Mezofilik Bacillus KW - İzolasyon KW - Alfa-amilaz KW - Termal stabilite KW - karakterizasyon CR - Referans1 Karademir G., Karademir B. Yem katkı maddesi olarak kullanılan biyoteknolojik ürünler, Lalahan Hayvancılık Araştırma Enstitüsü Dergisi 2003; 43(1): 61-74, 2003. CR - Referans2 Nelson DL., Cox MM. Lehninger principles of biochemistry, Chapter 6, W.H. Freeman and Company, 4th Ed. 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UR - https://doi.org/10.47495/okufbed.824607 L1 - https://dergipark.org.tr/tr/download/article-file/1392302 ER -