@article{article_827228, title={Genotoxic Evaluation of Bentazone and Chloridazon Herbicides in Eisenia hortensis Coelomocytes}, journal={Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi}, volume={20}, pages={963–967}, year={2020}, DOI={10.35414/akufemubid.827228}, author={Ulukütük, Sevgi and Ciğerci, İbrahim Hakkı}, keywords={Bentazon, Chloridazon, Eisenia hortensis, Genotoksisite, Comet yöntemi, Mikronukleus}, abstract={In current study, genotoxic effects of Bentazone and Chloridazon herbicides on Eisenia hortensis species were investigated. The species of E. hortensis were collected from the natural habitats in Afyonkarahisar. Comet assay and Micronucleus test was conducted to evaluate the genotoxicity in earthworm coelomocytes. The LD50 value of Bentazone herbicides was noted as 236 ppm and 76.6 ppm for Chloridazon, respectively. Then LD50/2, LD50 and 2XLD50 concentrations of Bentazone and Chloridazon herbicides were applied to E. hortensis for 48 h. Concentration dependent increase in DNA and chromosomal damage was observed (P < 0.05) by both herbicides. Highest DNA damage and micronucleus formation were noticed at highest doses compared to other concentrations and control group. It was concluded that Bentazone and Chloridazon induced DNA damage and chromosomal aberrations failure in E. hortensis earthworms.}, number={6}, publisher={Afyon Kocatepe Üniversitesi}, organization={Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi}